Sterilization Guidelines

Introduction
Equipment Classification
Levels of Disinfection
Cleaning/Reprocessing of GI Endoscopes
Glutaraldehyde
Methods of Sterilization and Disinfection


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Yale New Haven Hospital
QISS
GB 325
New Haven, CT
06504 USA

Dr. Jeff Topal
688-4634




Cleaning and Reprocessing of Flexible GI Endoscopes, pg3
3)  Cleaning
Mechanical cleaning is the first and most important step in removing the microbial burden from an endoscope. Retained debris may inactivate or interfere with the capability of the active ingredient of the high level disinfectant to effectively kill and/or inactivate microorganisms. Cleaning gastrointestinal endoscopes is necessary before automated or manual disinfection.
Fill a sink or basin with freshly made solution of water and a low-sudsing enzymatic detergent compatible with the endoscope.
Dilute according to the detergent manufacturer’s instructions.
Depending on the detergent formulation used, water temperature may be essential to activate the detergent solution.
Use fresh detergent solution for each endoscope to prevent cross contamination.
Low-sudsing detergents are recommended so that the device can be clearly visualized during the cleaning process to preclude personnel injury and to allow for complete cleaning of lumen surfaces. Excessive sudsing can inhibit good fluid contact with the device surfaces.
Immerse the endoscope.
Wash all debris from the exterior of the endoscope by brushing and wiping the instrument while submerged in the detergent solution. Whenever practical, leave the endoscope submerged in the detergent solution when performing all subsequent cleaning steps.
The instrument should be left under water during the cleaning process to prevent splashing of contaminated fluid.
Detach the suction and air/water valves, the biopsy channel cover, the distal end hood, if present, and all other removable parts. Discard those parts that are designated as disposable.
The endoscope must be completely disassembled so that all surfaces may be reached for thorough cleaning.
Use a small, soft brush and/or lint-free cleaning swab to clean all removable parts including inside and under the suction valve, air/water valve, and biopsy-port cover and openings.
Use of nonabrasive and lint-free cleaning tools will prevent damage to the endoscope.

Last modified: March 12, 2001.



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