Sterilization Guidelines

Introduction
Equipment Classification
Levels of Disinfection
Cleaning/Reprocessing of GI Endoscopes
Glutaraldehyde
Methods of Sterilization and Disinfection


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Yale New Haven Hospital
QISS
GB 325
New Haven, CT
06504 USA

Dr. Jeff Topal
688-4634




Cleaning and Reprocessing of Flexible GI Endoscopes, pg2
Cleaning the Endoscope in the Reprocessing Area
Have the following available:
Personal protective equipment (gloves, eye protection, impervious gown).
Leakage tester equipment.
Channel cleaning adapters (per manufacturer’s instructions).
Large basin of enzymatic detergent solution prepared according to the manufacturer's instructions.
Channel cleaning brushes and lint-free cleaning swabs.
Sponge and/or lint-free cloth.

2)  Leak Testing
Leak test the endoscope following manufacturer’s instructions.
Attach the leak tester and pressurize the scope before submerging it in water.
Remove the detachable parts of the endoscope.
With the pressurized endoscope completely submerged, flex the distal portion of the scope in all directions. Observe the insertion tube, distal bending section, and the universal cord for bubbles coming from the interior of the scope.
The leak test will detect damage to the interior or exterior of the endoscope. The leak test is done before immersion of the endoscope in reprocessing solutions to minimize damage to parts of the endoscope not designed for fluid exposure.
Follow the manufacturer’s instructions if a leak is detected or the endoscope appears damaged.

Last modified: March 12, 2001.



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