Sterilization Guidelines

Introduction
Equipment Classification
Levels of Disinfection
Cleaning/Reprocessing of GI Endoscopes
Glutaraldehyde
Methods of Sterilization and Disinfection


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Yale New Haven Hospital
QISS
GB 325
New Haven, CT
06504 USA

Dr. Jeff Topal
688-4634




Cleaning and Reprocessing of Flexible GI Endoscopes
1)  Preparing the Endoscope for Cleaning:
The initial steps in the reprocessing protocol begin in the procedure room immediately after removal of the insertion tube from the patient and before removing the endoscope from the power source.
Have the following available:
Personal protective equipment (gloves, eye protection, face protection and impervious gown).
Container with enzymatic detergent solution.
Sponge or soft, lint-free cloth.
Air and water channel cleaning adapters per manufacturer’s instructions.
Protective video caps.
Immediately after removing the endoscope from the patient, wipe the insertion tube with a wet cloth or sponge soaked in the freshly prepared enzymatic detergent solution.
Dispose of the cloth or sponge between cases.
Place the distal end of the endoscope into the enzymatic detergent solution. Suction the solution through the biopsy/suction channel until the solution is visibly clean. Alternate suctioning detergent solution and air several times. Finish by suctioning air.
Alternate suctioning of fluid and air is more effective than suctioning fluid alone in the removal of debris from internal lumens.
Immediate flushing of the biopsy/suction and air/water channels precludes drying of organic and inorganic debris on lumen surfaces and may remove large numbers or microorganisms.
Blow out air and flush water channels in accordance with the endoscope manufacturer's instructions.
Detach the endoscope from the light source and suction pump.
Attach protective video cap.
Transport the endoscope to the reprocessing area in a leak proof enclosed container.
Containers, sink, and basins should be large enough that the endoscope will not be damaged by being coiled too tightly.
A container will prevent contamination during transport.
Reprocessing should occur in a room separate from the procedure room and in a dirty utility room.


Last modified: March 12, 2001.



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