Veterinary Clinical Services

Preventive Medicine

Nonhuman Primate Quarantine

Protocol for Unconditioned and Conditioned Dogs and Long Term Maintenance of Dogs

UNCONDITIONED STATUS

1. Random Source (received at YARF only)
   1. On Arrival
      1. Review accompanying health certificate.
      2. VCS tech: Physical Exam - observe general condition, temperament; accept or reject.
      3. YARC tech: Dip for ectoparasites using Paramite (mix according to package directions)
      4. Submit blood sample for heartworm antigen test (3ml red top tube)
              If positive VCS will notify Veterinarian
      1. Veterinarian will contact vendor for credit
      2. Veterinarian give instructions for euthanasia

Dog may be released any time after this as "unconditioned"

DOG MUST BE USED ACUTELY BY DAY 7. ON DAY 8 IF DOG IS STILL HAS NOT BEEN USED, CONDITIONING WILL COMMENCE AT PI EXPENSE. PI WILL BE CHARGED FOR A FULLY CONDITIONED ANIMAL FROM THIS POINT ON

CONDITIONED STATUS

1. Random Source (received at YARF only)
   1. On Arrival
      1. Review health certificate
      2. VCS tech: Physical Exam - observe general condition, temperament; accept or reject.
      3. Submit blood sample forCBC/CS and heartworm antigen test
      4. Get fecal sample #1
      5. YARC tech: Dip for ectoparasites using Paramite (mix according to package directions)
      6. VCS tech: Immunize with Duramune DA2PP+CVK/LCI and Rabvac 3 or Imrab3
   2. Days 2 , 3 and 4
      YARC tech: Medicate daily with Panacur for treatment of internal parasites
   3. Days 12
      VCS tech:
      1. Physical Exam
      2. Submit fecal sample #2 for parasitology
   4. Day 14
      1. Boost with Duramune DA2PP+CVK/LCI
      2. Submit fecal #3 for parasitology

   * If any fecals are positive, contact veterinarian on service for treatment instructions.
      Dog must have 3 negative fecals before release as "conditioned"

2. Purpose Bred dogs (may be received into animal unit directly)
   1. On Arrival
      1. Physical Exam - report any abnormalities to veterinarian
      2. Review health and vaccination history
      3. Submit fecal sample for parasitology
         1. If positive, treatment options must be discussed with investigator
   2. Vaccinations continued depending on vaccination schedule at the vendor (puppies should receive Duramune DA2PP+CVK/LCI at 8,10 and 12 weeks or 9, 11 and 13 weeks and Rabvac 3 between weeks 12 to 14)
3. Long Term Maintenance
   1. Every 6 months
      1. Submit fecal sample for parasitology.
         1. Treatment as indicated, after consultation between VCS and investigator.
   2. Annually
      1. Vaccinate with Duramune DA2PP+CVK/LCI.
      2. Submit blood sample for heartworm antigen test (1-3ml red top),(see D.below)
   3. Triennially
      1. Vaccinate with Rabvac 3.
   4. Heartworm Prevention
      1. Test for heartworm antigen every spring in April/May
      2. If negative, give Heartgard Plus on the first of every month, May through November
         1. NOTE: beagles on hookworm study must not be given Heartgard
      3. Dogs that have undergone experimental procedures should be given Heartgard Plus only after obtaining the investigator’s permission; if Heartgard is to be withheld, a notation should be made by VCS in the dog’s record and on it’s cage card
4. Appendix
   1. Drugs and Vaccines utilized in protocol
      1. Duramune DA2PP+CVK/LCI: Canine Distemper, Adenovirus Type 2, Corona, Parainfluenza, Parvo;modified live and killed
      2. Rabvac 3: Rabies, killed or Imrab 3
      3. Panacur: Fenbendazole
      4. Paramite: Enmercaplomethyl phtalamide dimethylphosphorodiphthlate
      5. Heartgard Plus: Ivermectin/Pyrantel
      6. Nemex: Pyrantel pamoate

ECW/GC 97 amended 98+99 TAF/FH

Protocol for Quarantine, Conditioning and Long-Term Maintenance of Cats

1. Purpose Bred
   1. On Arrival (may be received directly into animal- unit or YARF)
      1. Non-pregnant Adults
         1. Physical exam - YARC/VCS technician will evaluate general condition and report any problems to veterinarian on service
         2. Submit fecal sample for parasitology, treat as needed
         3. Immunize with Eclipse IV and Rabvac 3
      2. Pregnant Females
         1. Physical exam -YARC/VCS technician will evaluate general condition and report any problems to veterinarian on service
         2. Submit fecal sample for parasitology; if treatment necessary check with veterinarian regarding when to institute and medication to use
         3. DO NOT VACCINATE until kittens are born and weaned or used acutely.
            At that time vaccinate mother with Eclipse IV and Rabvac 3
      3. Kittens (born at Yale or purchased)
         1. Physical exam -YARC/VCS technician will evaluate general condition and report any problems to veterinarian on service
         2. 9 and 12 weeks - submit fecal sample for parasitology, treat as needed
         3. 9 weeks -immunize with Eclipse IV
         4. 12 weeks-immunize with Eclipse IV and Rabvac 3.
2. Long Term Maintenance
   1. Every 6 months
      1. Submit fecal sample for internal parasites and treat as necessary
   2. Annually
      1. Vaccinate with Eclipse IV and Rabvac 3
         1. EXCEPTION: Do not vaccinate any female that may be pregnant until confirmed non-pregnant or kittens have been weaned.
3. Appendix and Notes
   1. At Liberty, which is our current vendor for purpose-bred cats, animals receive
      Felovax (killed) vaccine annually if non-pregnant, and do not receive any Rabies vaccinations
   2. YARC does not currently purchase random source cats. Should this change, an addendum to this protocol will be written.
   3. Drugs and Vaccines Used In Protocol
      1. Eclipse IV: Feline Rhinotracheitis, Calici, Panleukopenia,Chlamydia psittaci; modified live
      2. Rabvac 3: Rabies; killed
      3. Pipcide: Piperazine citrate
      4. Task: Dichlorvos
      5. Paramite: Enmercaptomethyl phthalamide dimethylphosphorodiphthylate
      6. Canex: Rotenone

ECW/GC 1997

Protocol for Quarantine, Conditioning and Long-Term Maintenance of Ferrets

1. Adults
   1. On Arrival
      1. Adult ferrets arrive from the vendor (Marshall Farms) fully vaccinated for rabies and distemper.
2. 8-10 weeks of Age
   1. On Arrival
      1. Most animals have received vaccinations for distemper and rabies at the vendor.
         Check accompanying health certificate to verify (this could vary dependent on the actual age of animals at shipping time).
   2. 3 Weeks After Arrival
      1. Vaccinate with Galaxy D
      2. Vaccinate with Imrab 3 (if not given Rabies vaccine prior to shipment)
3. Long Term Maintenance
   1. Every 6 Months
      1. Submit fecal sample for parasitology.
         1. Treat if indicated
   2. Annually
      1. Vaccinate with Galaxy D and Imrab 3.
4. Notes
   1. Drugs and Vaccines utilized in protocol
      1. Galaxy D: Canine Distemper, modified live
      2. Imrab 3: Rabies, killed

ECW/GC 1997

Protocol for Conditioning of Pigs

1. It is recommended that pigs will be allowed to acclimate for at least 5 days following shipment and before experimental use.
2. The investigator may request (at the time of ordering) that the pigs be placed on antibiotics upon arrival. The request will be relayed to YARF personnel by the animal buyer. The treatment will be given for the first 5-7 days.
   Either of two treatment regimens may be used:
   1. Mix 1 gram of Tylosin powder into 20 pounds non-pelleted food and feed as usual. Continue for 7 days or until pigs are used, whichever is shorter.
   2. Each pig can receive 1000mg Tetracycline once/day.
      Mix 4x250mg or 2x500mg capsules into individual animal's feed
      Open capsules, dump powder into feed and mix - wear gloves while handling medication.
3. Any abnormalities, such as nasal discharge, diarrhea, loss of appetite, etc. will be reported to VCS.

ECW10/97

Rabbit Conditioning and Enrichment

Arriving Rabbits:

• Day 0 (day of arrival): No pellets, one small handful of Hay
• Day 1: 25% of food ration, one small handful of hay
• Day 2: 50% of food ration, one small handful of hay
• Day 3: 75% of food ration, one small handful of hay
• Day 4:100% of food ration, one small handful of hay

Daily Maintenance Feeding Protocol for Rabbits:

Environmental Enrichment:

• Feed each rabbit a small handful of hay twice weekly, i.e. two evenly spaced days such as Monday and Thursday
• Rabbits may have 1/4 slice of apple or a 2-3 inch slice of carrot once weekly alternate to hay feeding days. Rabbits may be hand fed 2-3 Cheerios or Bite-sized Shredded Wheat weekly as a food treat. This interaction with the animal technician will provide the opportunity for close examination of each rabbit and will confirm normal posturing and movement.
• Toys will be rotated throughout all the rabbit's cages.
• Colored star stickers placed onto the cage card are used to identity rabbits which enjoy attention and being stroked and petted.

Health Monitoring:

• All food intake and stool production should be observed daily. Rabbits with significant decrease in food intake, decrease in stool production, or change in stool consistency should be reported to Veterinary Clinical Services (VCS).
• VCS will advise YARC when it may be necessary to monitor body weight by weighing and adjusting food consumption.

Reference:

The Biology of the Laboratory Rabbit 1994; Manning, Ringler, and Newcomer

pls 5/98

Outline of Sheep Flock Maintenance

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Copyright © 1999 Yale Animal Resources Center, Yale University
Last modified: February 13, 2000