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Veterinary Clinical Services


Euthanasia

Euthanasia Guidelines     Recommended Euthanasia Methods


Euthanasia Guidelines

 

Yale University Policy on Humane Euthanasia of Laboratory Animals

The Yale Animal Resources Center (YARC) provides a euthanasia service. Animals must be euthanized using acceptable humane standards as described in the 2000 Report of the AVMA Panel on Euthanasia. The YARC requires investigators to legibly complete a Euthanasia Cage Card for each animal or cage of animals to be euthanized. Every animal must have access to a food and water source until euthanized.

In certain circumstances, the veterinarian may use their professional judgement determine a time within which euthanasia must be performed. Veterinary Clinical Services (VCS) will discuss this with the investigator. However, if humane considerations are paramount and VCS cannot reach the investigator or the animal(s) have yet to be euthanized within the designated time, the clinician will order euthanasia. VCS will inform the Director of YARC, the Manager of Regulatory and Safety Services, and the Director of YACUC of the specific circumstances and that VCS has ordered euthanasia.

Addendum:

  1. YARC staff can only euthanize animals in a cage with a signed euthanasia card attached to it. All animals in that cage will be euthanized.
  2. If VCS feels that an animal should be euthanized for medical or humane reasons, the investigator will be contacted. If the investigator agrees to euthanize the animal he/she will separate the sick animal and fill out and sign the euthanasia card.
  3. If the investigator is unable to fill out the card in a timely fashion, VCS will fax a copy of the cage card to them (to avoid any question which animal is being discussed) and have them fax it back with their signed permission to euthanize. It this is not possible (e.g. weekends) VCS can offer to fill out the card for them. Based on verbal or written consent from the investigator a VCS technician or clinician must separate the sick animal(s) into a separate cage and fill out and sign the euthanasia card. Document the investigator communication, including  time, date, name of authorizing person, on the euthanasia card and in the VCS sick report or animal record.
  4. If an investigator cannot be reached or is unwilling or unable to agree to the euthanasia, a VCS clinician may order euthanasia. The clinician will have to base his/her decision on a personal examination of the animal(s) in question. The clinician will have to sign the euthanasia card and make sure that the animal(s) are separated out properly. The approved protocol should be consulted  because some protocols have death approved as an endpoint.

AVMA Recommended Methods

Amphibians - Birds - Cats - Dogs - Fish - Horses - Marine mammals - Mink, fox and other mammals produced for fur - Non-human primates - Rabbits - Reptiles - Rodents and other small mammals - Ruminants - Swine - Zoo animals

Species Acceptable Conditionally acceptable
Amphibians Inhalant anesthetics
CO
CO2
barbiturates
tricaine methanesulfonate
double pithing
benzocaine
Pithing
gunshot
penetrating captive bolt
stunning and decapitation
decapitation and pithing
Birds Inhalant anesthetics
CO
CO2
barbiturates
N2
Ar
cervical dislocation*
decapitation
Cats Inhalant anesthetics
CO
CO2
barbiturates
N2
Ar
Dogs Inhalant anesthetics
CO
CO2
barbiturates
N2
Ar
electrocution
penetrating captive bolt
Fish Tricaine methanesulfonate
benzocaine
barbiturates
Stunning and decapitation
decapitation
Horses Barbiturates
chloral hydrate
chloral hydrate/MgSO4/pentobarbital
Penetrating captive bolt
gunshot
electrocution
Marine mammals Barbiturates
etorphine hydrochloride
Succinylcholine chloride and potassium chloride
gunshot
Mink, fox and other mammals produced for fur Inhalant anesthetics
CO
CO2
barbiturates
N2
Ar
electrocution followed by cervical dislocation
Non-human primates Barbiturates Inhalant anesthetics
CO
CO2
N2
Ar
Rabbits Inhalant anesthetics
CO
CO2
barbiturates
N2
Ar, cervical dislocation
decapitation
penetrating captive bolt
Reptiles Barbiturates
inhalant anesthetics
CO2
Gunshot
penetrating captive bolt
stunning and decapitation
decapitation and pithing
Rodents and other small mammals Inhalant anesthetics
CO
CO2
microwave irradiation
barbiturates
N2
Ar
cervical dislocation *
decapitation
Ruminants Barbiturates Penetrating captive bolt
gunshot
electrocution
chloral hydrate
Swine Barbiturates
CO2
Inhalant anesthetics
CO
penetrating captive bolt
gunshot
electrocution
chloral hydrate
Zoo animals Inhalant anesthetics
CO2
CO
barbiturates
N2
Ar
penetrating captive bolt
gunshot

* requires prior anesthesia unless scientifically justified and approved by the IACUC.

 

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Copyright © 1999 Yale Animal Resources Center, Yale University
Last modified: March 27, 2006