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Keck
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Protein Chemistry >
Peptide HPLC
Peptide HPLC: Analytical/Without
Fraction Collection
Automated peptide mapping by C-18 reverse-phase HPLC is
available on an HP1090 system that has been optimized for
high sensitivity HPLC. Digests are loaded in 50 µl of a
suitable solvent, (which should not contain detergent) and
should contain from 5 to 100 pmol protein. Unless otherwise
requested, peptides will be detected by their absorbance at
210 nm.
Peptide HPLC:
Preparative/With Fraction Collection
Protein digests that contain from 5 to
100 picomoles in a volume of 50 µl may be fractionated by C-18
reverse-phase HPLC and automatically collected with peak detection into
100-150 individual Eppendorf tubes. If further purification is
required, individual peaks collected from an HPLC collection run of a
protein digest can be re-chromatographed on an Aquapore C-8 column.
Cerenkov counting, which can detect high energy beta emitters like
[32P] without having to add scintillation
fluid to the sample, of up to 100 HPLC fractions from a single
collection run is also available. (More
Information)
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