General Information:

Genomic Services:

Proteomic Services:

Keck Home Page >  Service Charges

Service Charges
(As of 05/02/2008)

GENOMIC SERVICES:
· Microarrays
· DNA Sequencing
· Oligonucleotide Synthesis

Total RNA Q.C. only (nanodrop & bioanalyzer)

$15

$18

AFFYMETRIX

Expression Full Service

$350

$400

2-cycle Full Service

$500

$550

Exon Full Service

Gene Array Full Service

$210

$240

Prelabeled cRNA Hyb Only

$175

$192

EXIQON

miRNA Expression Profiling Full Service

$210

$230

ILLUMINA

Expression Full Service Per Sample

$150

$165

Prelabeled cRNA Hyb Only Per Chip

$80

$90

NIMBLEGEN

Expression Full Service

$400

$440

Expresssion Hyb Only

$145

$160

SEQUENOM

Expression Full Service (pricing based on the size of project)

SPOTTED

Spotted Catalog Arrays (see list)

Expression Full Service

$240

$265

Expresssion Hyb Only

Scan Only

$25

$30

Raw Data Analysis using GenPix software

$97 / hr

$112 / hr

Custom Microarray (pricing based on the size of project)

Cluster Generation + sequencing (includes reagents and labor)

Library preparation

Genomic DNA Q.C. only (spectrophotometer & agarose gel)

AFFYMETRIX

Genotyping Full Service

Genotyping Hyb Only

$70

$80

ILLUMINA

Genotyping Full Service Per Sample

$110

$120

Golden Gate Full Service Per 96 Samples

$960

$1060

NIMBLEGEN

Genotyping Full Service

$275

$305

Genotyping Hyb Only

$145

$160

SEQUENOM

Geontyping Full Service (pricing based on the size of project)

ABI 7900HT Realtime PCR Plate Run (equipment use only)

ABI 7900HT SNP Plate Run (including real time run and post read)

ABI 7900HT SNP Plate Post Read Only

Please refer to the Keck Biostatistics Department by contacting Amy by e-mail.

BeadStudio, GCOS, GenePix, GeneSpring, NimbleScan, SDS Workstations

GCOS or BeadStudio Software Tutorial (30 minutes)

Premixed Reactions

Premixed Reactions
(Incorrect Tube Submission)

High Volume 96 well sequencing

48 - 79 samples per plate

80 - 96 samples per plate

Primer Walking

Sequence runs each

Primers as needed for primer walking

Consensus Sequence Assembly and Editing

Difficult templates
(includes BAC, 70% GC rich, hairpin structures that require altered sequencing methods)

Pyrosequencing

Assay Design

Pyrosequencing Production Genotyping

No PCR

With PCR setup

40 nanomole scale (1-60 bases, unmodified)

Setup charge

Residue charge

40 nanomole scale (>60 bases with A,G,C or T @ 3' end)

Setup charge

Residue charge

0.2 micromole scale (A,G,C or T @ 3' end, LV200 nanomole)

Setup charge

Residue charge

0.2 micromole scale (non-standard 3' end)

Setup charge

Residue charge

1.0 Micromole Scale

Setup charge

Residue Charge

Gel purify from 40 nanomole scale

Surcharge per oligo

Gel purify from 0.2 micromole (LV200 nanomole)

Surcharge per oligo

Gel purify from 1.0 micromole scale

Surcharge per oligo

RNA/2'-O-Methyl RNA

1.0 Micromole Scale

Setup charge

Residue charge

Consulting Charge (per hour)

Hydrolysis

Oxidation

Analysis

Tryptophan/MSA Hydrolysis

HPLC SEC/Laser Light Scattering
(Add Set-up Surcharge below)

Standard Running Buffer Set-up Surcharge

Customer Requested Running Buffer Set-up Surcharge

SLM 8000C Spectrofluorometer
(up to 4 hours/day)

Spectrofluorometer
6 hours/day

Spectrofluorometer
9 hours/day

Stopped-Flow Spectrofluorometer

Stopped-Flow Spectrofluorometer Training

Open Access Dynamic Light Scattering Instrument

Open Access DLS Training

Isothermal Calorimetry (ITC)

ITC 2-day training course

SPR Service

DIGE service with Cy-labeling of 3 samples only

DIGE service with Cy-labeling of 2 samples only

DIGE service with Cy-labeling of 3 samples (double sample + dye)

DIGE service with Cy-labeling of 2 samples (double sample + dye)

DIGE service beginning with SDS PAGE (12.5% polyacrylamide)

Gel Scanning + Preliminary Data Analysis

Gel Picking of up to 96 user-selected spots

Primary Protein Identification (per spot)

Secondary Protein Identification (per spot)
(Done manually after primary protein identification)

Albumin/IgG depletion from serum samples

Fluorescent scan on the Typhoon scanner
(per 10 minute scan)

DIGE Clean-up

ICAT-Based MS/MS Protein Profiling

iTRAQ Differential Protein Expression Analysis Labeling (2-plex)

iTRAQ Differential Protein Expression Analysis Labeling (3-plex)

iTRAQ Differential Protein Expression Analysis Labeling (4-plex)

MudPIT (per fraction cost 1-14 fractions)

MudPIT (15-20 fractions)

Digest

Enzymatic/CnBr Digest

"In gel" or Robotic Digestion
(must submit 8 or more samples)

HPLC

Analytical

Preparative/Digest

Preparative/Digest (radioactive)

Preparative/Repurification

CEX/MudPIT

2DLC ¹

Avidin (1 fraction)

Avidin (20 fractions)

FT-ICR Mass Spectrometry

NanosprayESI FT-ICR

ESI Exact/accurate Mass

ESI IRMPD MS/MS

ESI ECD MS/MS

Online LC-MS

Custom Analysis

MALDI Mass Spectrometry

MALDI

MALDI (Linear + Reflectron)

MALDI (Digest)

MALDI (Biomarker)

MALDI (Tof-Tof)

MALDI (Tof-Tof MS/MS)

MALDI (Misc)

Electrospray Mass Spectrometry

ESMS on Micro

LC MS/MS automated Mascot Protein Identification (digest charge is not included)

LC MS/MS : Raw data provided only, no interpretation of data.  (Suitable for >100 fmoles of sample, digest charge is not included)

Nanospray MS/MS Protein Identification (digest charge is not included)

ZipTip Desalting

set-up charge

per peak charge

Residues 1-20

Residues 21-51

Residues 51 +

Amino-terminal Acetylation

His/Trp/Cys

Cleavage/Purification/MS/AAA ²

Resin Split

25 µmole Scale

Crude, 5-25 residues, 5-50 mg

Purified, 5-25 residues, 1-10 mg 

50 µmole Scale

Crude, 5-25 residues, 10-100 mg

Purified, 5-25 residues, 2-20 mg 

100 µmole Scale

Crude, 5-25 residues, 20-200 mg

Purified, 5-25 residues, 4-40 mg

Residues 1-5

Residues 6 - End

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Last modified: 20-Dec-2006 (GB)