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Keck
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RNA & 2'O-methyl RNA
RNA & 2'O-methyl RNA
We synthesize both RNA and 2'-O-methyl RNA only
at the 1.0 µmole scale because 40 nanomole columns are not available and because
the 0.2 µmole scale uses only about 15% less reagent than the 1.0 µmole. For RNA
oligos longer than 40 bases, we use TOM-protected RNA monomers which allow for
increased chain elongation. For RNA oligos 40 bases or less, we use the standard
TBDMS-protected RNA monomers. In addition, we supply all RNA with a 2'
protecting group (TOM or TBDMS) which must be removed by the researcher before
use. Click
HERE for the
protocols. We remove all protecting groups from 2'-O-methyl RNA.
Turnaround times for RNA are longer than for DNA because we try to accumulate enough
orders to use these expensive monomers efficiently.
Chimers of DNA/RNA (or 2'-O-methyl RNA) are also available. The charge is simply the
sum of the per-residue charges for the numbers of each type of monomer incorporated, plus
the setup charge for the type of monomer at the 3' end. These orders may be submitted via
the Order
Modified Oligos site.
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