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Oligo Synthesis >
Modifier Pricing
MODIFIER PRICING
There are three factors in determining the cost of a modified oligo:
The Synthesis Cycle to be Used:
Determining the cost of a modified oligo begins
with selecting the scale of the synthesis and then calculating the setup charge
and the per- residue charge for that scale. A normal DNA setup charge is used
unless there is an RNA or 2'-O-methyl RNA residue at the 3' end for which those
setup charges would apply. A normal DNA per-residue charge is used for the
modified residues unless those modifiers are RNA or 2'-O- methyl RNA. Please see
our price schedule and
Oligo Cost Calculator for
normal setup and per-residue charges.
Most of our specialy
reagents are ordered from
Glen Research. Glen offers only 0.2
micromole and 1.0 micromole columns for initiating syntheses with 3' modified residues (40 nanomole columns are not available). Therefore, the Normal 0.2 µm and Normal 1.0 µm
synthesis cycles are used for all syntheses with modified 3' residues.
The LV (low volume) 40 nm, LV 0.2 µm, or Normal 1.0 µm cycles are used for all
syntheses with unmodified 3' DNA residues. The LV cycles use approximately 70%
less reagent than the Normal 0.2 µm synthesis cycle.
The Wide Pore 1.0 µm cycle is used for all 1.0 µm oligos
over 50 bases in length.
Phosphorothioate linkages will be charged at $5.75
per linkage ($6.61 for non-Yale orders) to cover additional labor in setting up
and maintaining the synthesizer.
The Number of Additions per Bottle:
Glen generally offers specialty reagents in 50 micromole, 100 micromole, and 0.25 gram
bottles.
The number of additions available per bottle varies with each cycle and is displayed
below:
|
50 micromole bottle
|
LV 40 nm cycle: |
7 additions |
LV 0.2 µm cycle: |
5 additions |
Normal 0.2 µm cycle:
|
2 additions |
Normal 1.0 µm cycle:
|
2 additions |
|
100
micromole bottle
|
LV 40 nm cycle: |
16 additions |
LV 0.2 µm cycle: |
14 additions |
Normal 0.2 µm cycle:
|
5 additions |
Normal 1.0 µm cycle:
|
5 additions |
|
0.25 g
Bottle
|
The number of additions
per 0.25 g bottle varies with molecular weight. E-mail us at
oligos@yale.edu for more information. |
|
Pre-mixed 0.25 g "N" amidite (3.0ml)
or 3ml doped mixture
|
LV 40 nm cycle: |
48 additions |
LV 0.2 µm cycle: |
45 additions |
Normal 0.2 µm cycle:
|
17 additions |
Normal 1.0 µm cycle:
|
17 additions |
The Prices of Specialty Reagents:
In addition to the set-up and per-residue
charges, the Glen Research
or other company's
list price +15% for the needed amount of specialty amidites and/or columns along with any ancillary reagents will be applied. To
eliminate issues of stability of the unincorporated monomers, we special-order
the reagent for your oligos and discard any leftovers. We will work with you to
group your orders so as to minimize your cost by maximizing the utilization of
expensive monomers. If you prefer, we will work our way down a list of oligos
until a specialty reagent is exhausted.
Generally, we will incorporate ANY commercially
available modifier, not just those listed below, as long as it is
particulate-free. To avoid issues of improper storage and handling,
we recommend allowing us to order the modified phosphoramidite/column. However,
if you would like to provide us with a phosphoramidite/column that you purchased
or synthesized, a $250 surcharge for each different modification will apply.
This surcharge covers the additional labor costs of modified phosphoramidite/column
handling and storage, synthesizer programming, oligo processing and e-mail
correspondence. Also, since we are not supplying the modified phosphoramidite/column,
our usual quality guarantee does not apply and the oligos must be accepted "as
is". The following is a list of
commonly ordered specialty reagents and columns. Remember, we will incorporate
ANY commercially available modifier, not just those listed below, as long as it
is particulate-free.
|
UltraMild Reagents
|
Item |
Pack |
Price
|
dA ,dG, dC
columns |
0.2 um |
$11.50 ea. |
| 1.0 um |
$17.25 ea. |
| dA, dG, dC amidites |
0.25 gram |
$17.25 ea. |
| Special Cap A |
1 bottle |
$119.60 |
| potassium carb. |
1 bottle |
$34.50 |
|
Inosine
|
Item |
Pack |
Price
|
d-Inosine |
100 micromole |
$57.50 |
| |
0.25 gram |
$138.00 |
| |
0.2 µm CPG column |
$20.70 each |
| |
1.0 µm CPG column |
$34.50 each |
|
Chemical Phosphorylation
|
Item |
Pack |
Price
|
5'-Phosphate
|
100 micromole
|
$57.50
|
| |
0.25 gram
|
$184.00
|
| |
|
|
3'-Phosphate
|
0.2 µm CPG column
|
$17.25 each
|
| |
1.0 µm CPG column
|
$28.75 each
|
| |
|
|
Trityl ON 5'-Phosphate
|
100 micromole
|
$69.00 |
| |
0.25 gram
|
$230.00
|
|
Primary Amines and Thiols |
Item
|
Pack
|
Price
|
5'-Amino Modifier C3-TFA |
100 micromole
|
$57.50
|
| |
0.25 gram
|
$201.25
|
| |
|
|
5'-Amino Modifier C6-TFA
|
0.25 gram
|
$115.00
|
| |
100 micromole |
$34.50
|
| |
|
|
5'-Amino Modifier C12
|
100 micromole
|
$103.50 |
| |
0.25 gram
|
$345.00
|
| |
|
|
3'-PT-Amino Modifier C3 |
0.2 µm CPG column
|
$24.44 each
|
| |
1.0 µm CPG column
|
$40.25 each
|
| |
|
|
|
3'-PT-Amino Modifier C6 |
0.2 µm CPG column
|
$24.44 each
|
| |
1.0 µm CPG column
|
$40.25 each
|
| |
|
|
3'- Amino Modifier C7
|
0.2 µm CPG column
|
$24.44 each
|
| |
1.0 µm CPG column
|
$40.25 each
|
| |
|
|
Thiol Modifier C6 S-S
|
100 micromole
|
$172.50 |
| |
0.25 gram
|
$414.00
|
| |
|
|
3'- Thiol Modifier C3 S-S
|
0.2 µm CPG column
|
$21.56 each
|
| |
1.0 µm CPG column
|
$38.94 each
|
|
Dyes
|
Item
|
Pack
|
Price
|
Cy 3, Cy 5
(Requires
UltraMild
reagents)
|
50
micromole |
$218.50
|
| 100 micromole |
$402.50 |
| Cy 3.5, Cy 5.5
(Requires
UltraMild
reagents)
|
50 micromole |
$396.75 |
| 100 micromole |
$759.00 |
| TAMRA-dT
(Requires
UltraMild
reagents)
|
50 micromole |
$287.50 |
| 100 micromole |
$569.25 |
| 3'-TAMRA
(Requires
UltraMild
reagents)
|
0.2 um CPG-column |
$34.50 |
| 1.0 um CPG-column |
$57.50 |
| 5'-dabcyl |
50 micromole |
$143.75 |
| 100 micromole |
$258.75 |
| dabcyl-dT |
50 micromole |
$207.00 |
| 100 micromole |
$402.50 |
| 3'-dabcyl |
0.2 um CPG-column |
$34.50 |
| 1.0 um CPG-column |
$57.50 |
Fluorescein
|
50 micromole
|
$218.50 |
100 micromole
|
$339.25
|
0.25 gram
|
$684.25 |
3'-Fluorescein
|
0.2 µm CPG column
|
$34.50 each
|
1.0 µm CPG column
|
$57.50 each
|
5'-Fluorescein (6-FAM)
|
50 micromole
|
$126.50
|
100 micromole
|
$247.25 |
|
5'-Hexachloro-Fluorescein (HEX)
|
50 micromole
|
$218.50
|
100 micromole
|
$431.25 |
|
5'-Tetrachloro-Fluorescein (TET)
|
50 micromole
|
$207.00
|
100 micromole
|
$402.50 |
Acridine
(Requires
UltraMild
reagents)
|
50 micromole
|
$218.50 |
100 micromole
|
$339.25 |
0.25 gram
|
$776.25 |
3'-Acridine
(Requires
UltraMild
reagents)
|
0.2 µm CPG column
|
$34.50 each
|
1.0 µm CPG column
|
$57.50 each
|
Etheno-dA
(Requires
UltraMild
reagents)
|
100 micromole
|
$120.75 |
0.25 gram
|
$293.25 |
Fluorescein-dT
|
50 micromole
|
$207.00 |
100 micromole
|
$373.75 |
0.25 gram
|
$776.25 |
|
Halogenated deoxy and ribonucleotides
|
Item |
Pack |
Price
|
5-Bromo-dC
5-Bromo-dU
5-Iodo-dU |
100 micromole
|
$69.00
|
| |
0.25 gram
|
$184.00 |
| |
|
|
5-Iodo-dC
5-Fluro-dU |
100 micromole
|
$155.25 |
| |
0.25 gram
|
$408.25 |
| |
|
|
3' 5-Bromo-dU
|
0.2 µm CPG column
|
$34.50 each
|
| |
1.0 µm CPG column
|
$57.50 each
|
| |
|
|
Bromo-rU
Iodo-rU |
100 micromole
|
$224.25 |
| |
0.25 gram
|
$546.25 |
|
Spacers
|
Item |
Pack |
Price
|
Spacer 9
Spacer C3 |
100 micromole
|
$86.25 |
| |
0.25 gram
|
$276.00 |
| |
|
|
Spacer 18
|
100 micromole
|
$109.25 |
| |
0.25 gram
|
$276.00 |
| |
|
|
d-Spacer
|
100 micromole
|
$94.75 |
| |
0.25 gram
|
$339.25 |
| |
|
|
3' Spacer C3
|
0.2 µm CPG column
|
$17.25 each
|
| |
1.0 µm CPG column
|
$28.75 each
|
|
Biotin
|
Item |
Pack |
Price
|
Biotin-TEG
|
50 micromole
|
$218.50
|
| |
100 micromole
|
$339.25 |
| |
0.25 gram
|
$776.25 |
| |
|
|
5'-Biotin
|
50 micromole
|
$143.75 |
| |
100 micromole
|
$258.75 |
| |
0.25 gram
|
$747.50 |
| |
|
|
3' Biotin-TEG
|
0.2 µm CPG column
|
$34.50 each
|
| |
1.0 µm CPG column
|
$57.50 each
|
| |
|
|
Biotin-dT
|
50 micromole
|
$207.00 |
| |
100 micromole
|
$373.75 |
| |
0.25 gram
|
$776.25 |
|
Reverse Synthesis
|
Item |
Pack |
Price
|
dA-5'
dG-5'
dC-5'
dT-5'
|
0.25 gram
|
$86.25
|
| |
|
|
dA-5'
dG-5'
dC-5'
dT-5'
|
0.2 µm CPG column
|
$21.57 each
|
| |
1.0 µm CPG column
|
$28.75 each
|
|
Branches
|
Item |
Pack |
Price
|
|
Symmetric Doubler |
100 mg
|
$172.50
|
250 mg |
$276.00 |
|
Asymmetric Doubler |
100 mg
|
$207.00
|
250 mg |
$345.00 |
|
Trebler |
100 mg
|
$207.00
|
250 mg |
$345.00 |
|
Chain Terminators
|
Item
2',3'-ddC only!
(ddA, ddG and ddT columns are not available) |
Pack |
Price
|
3'-dA
3'-dG
3'-dC
3'-dT |
0.2 µm CPG column
|
$57.50 each
|
| |
1.0 µm CPG column
|
$172.50 each
|
|
Cross-linking
|
Item |
Pack |
Price
|
Psoralen C2
Psoralen C6 |
100 micromole
|
$224.25 |
| |
0.25 gram
|
$569.25 |
|
