HHMI Biopolymer/W.M. Keck Foundation Biotechnology Resource Laboratory at Yale University HPLC SEC Laser Light Scattering Determination of Native Protein Molecular Weights  

Name: Date:
Department: P.I.:
Yale Cancer Center Member?       Yes      No           (circle one) Telephone:
Institution: Fax:
Shipping Address: 
 
 
 		 Billing Address: (Required)                           (City, State, Zip 
 

 
e-mail: 
 
 
 		 Charge Number: 

(If HHMI enter HHMI and HHMI cost center number, otherwise enter Yale charge number, purchase order number or PHS record of call.)

Date Submitted _______________     Sample name (3 letter maximum)  ______  ______  ______

Sample concentration _______ mg/mL        Volume _______ mL   (Minimum sample amounts are 100 - 300 µg/run)

Sample concentration based on: _____________________________________________________
Hydrolysis/amino acid analysis is the recommended approach for determining protein concentrations.  This service can be carried out for an additional charge, see the listing of charges at   (http://info.med.yale.edu/wmkeck) .
Would you like to have amino acid analysis carried out on this sample?    Yes     No     (Please circle yes or no)

A280 ____________    A260 ____________       A280/A260 ____________

Sample buffer ___________________________________________________________________

Glycoprotein?     Yes     No                      Glycerol?    Yes _____%     No

Should a reducing agent (1 mM DTT) be included during the HPLC GPC run?     Yes      No

Is the sample stable at room temperature for 12 hours?     Yes     No
  (Note: please contact Ewa Folta-Stogniew via e-mail or phone (203) 737 43 87, at least 48 hours beforehand to ensure that sufficient instrument time will be available)

Swiss Assension Number: ______________   Sequence-Predicted Monomer MW:  ____________

Would you like the eluted protein collected (no fractionation, total volume ~25 mL - additional $20)?  Yes    No

Give the full name of the protein _____________________ What is the source? _____________
                (Note: the sequence-predicted monomer MW is requested to enable calculation of the MW error for the monomer) 

For additional information, please consult our Web Page: (http://info.med.yale.edu/wmkeck/6_16_98/Lsmemoa.htm) or contact Ewa Folta-Stogniew
Telephone: (203) 737-4387 Fax: (203) 785-6404 Attn: Ewa Folta-Stogniew  email: Ewa.Folta-Stogniew@yale.edu Samples accompanied by a completed sample submittal form should be addressed to:  

Mailing Address: Address for Courier Service:
Attn: Ewa Folta-Stogniew 
Yale University,  
MB&B Dept. 
333 Cedar Street 
New Haven, CT 06520-8024		 Attn: Ewa Folta-Stogniew
W.M. Keck Facility 
Yale University
333 Cedar St.
SHM I-140
New Haven, CT 06510