The Human oligo array is printed
with Telechem Stealth SMP3 pins in 32 pin conformation. The
OHU16K array has 24 columns and 22 rows with only 4 spots on
the 23 rd row in each of 32 subarrays. To aid analysis the
"grid" pattern for this array (.gps file) for Axon GenePix
Pro analysis is downloadable below:
MOUSE
ARRAYS
MM4.6K
ARRAYS-RETIRED
We have processed the
Research Genetics 5K sequence-verifed clone set to arrayable
PCR products. This link allows researchers to search the
Research
Genetics -
Mouse
5K clone list. in
Excel. A hyperlinked list of the MM4.6K slide is available
at the
Yale Microarray Database
and the set can be queried
here.
The MM-4.6K slide represents
the first 48 @ 96-well plates, 4,608 cDNAs, from the
Research Genetics 5K list. They are arrayed in a 4 X 4 array
with 24 X 24 spots in each subarray. Each cDNA is
represented by duplicate adjacent spots. The
center-to-center spot distance is 175 µm and the spots
are 125 µm diameter. There are two identical arrays on
each slide separated by sufficient space to hybridize each
array under a separate coverslip. At the first two positions
in each subarray there are cy3 and cy5- end labeled M13
oligonucleotide spots as "landing-lights".
The controls for both the
MM4.6K and MM4.6K-R are present as two lines of spots
separated from the array above sub-array one.
They are as
follows:
IMPORTANT - PLEASE NOTE: We recommend
that researchers re-sequence any clone/EST which yields
interesting expression patterns. There is an intrinsic level
of mismatch between clone assignment and actual identity in
the Research Genetics mouse clone set which may be anywhere
between 5-20%. We are in the process of resequencing
approximately 5% of the clones to assess the level of
mismatch in our particular replicate set. Please contact
Janet
Hager if you have
sequencing data done by others at Yale, or beyond, that you
are able to reciprocally share with us regarding the
Research Genetics
rg_mm_seq_ver_011100
set.
NIA15K
ARRAYS
We have
processed an
NIA
15K
mouse cDNA clone set which was made available to us from
NCI. The first releases of this slide are now available -
October, 2001. More information on the cDNA set can be found
here.
15,000 genes
per array -1 rep -1 array/slide
ARABIDOPSIS
ARRAYS
Arabidopsis
9.2K slides are available for Yale and non-Yale
investigators. 9,216 clones, single spot, 175 µm
center-to-center distance, 2 arrays per
slide.
