The Biophysical Resource

---

The Biophysical Resource is a facility operated by  Keck Foundation Biotechnology Resource Laboratory at Yale University.   We currently have resources to study oligomeric state of biomolecular assemblies, as well as thermodynamics and kinetics of macromolecular interactions using:  laser light scattering detectors: multiangle laser light scattering detector and dynamic light scattering detector (MALLS and DLS), isothermal microcalorimetry (ITC), surface plasmon resonance biosensor (SPR), stopped-flow spectrofluorometer, fluorescence polarization system, and fluorescence spectrophotometer.

Light Scattering Service

Description of the instrumentation

Sample requirements

Charges

Publications: reported SEC/LS data collected and analyzed through this service

Invited talks

 

Expansion of Light Scattering Service:

   DAWN HELEOS II multiangle laser light scattering detector and new refractive index detector:OPTILab rEX.

     The second SEC/LS system is capable of determination of MW for a 40 kDa protein at the concnetration from ~1 ug/ml to ~20 mg/ml.

     Variable temperature option (from 4C to 50C) coming in June.

   Eclipse 3; a state-of-the-art separation system that uses the asymmetric flow Field Flow Fractionation (AFFF) technique.

 

Dynamic Light Scattering Instrumentation

Description of the instrumentation

Schedule

Charges

Isothermal Calorimeter  (ITC)

Description of the instrumentation

Schedule

Charges

Spectrofluorometer

Description of  the instrumentation

Schedule

Charges

Stopped-Flow Spectrofluorometer

Description of the instrumentation

Schedule

Charges

Surface Plasmon Resonance- BiaCore Biosensor

Description of the instrumentation

Schedule

Charges

Fluorescence Polarization System

"Open Access" Instrumentations schedule

Stopped-flow_log
ITC_log
DLS_log
Fluorometer_log

The results collected through the services provided by the Biophysics Resource contributed to at least 53 publications since 1998
(sorted by technology and listed in chronological order)

Spectrofluorometer	ITC	Stopped-Flow  Spectrofluorometer	Fluorescence  Polarization System	Light Scattering  Service	SPR BiaCore	Keck's Facility Front Page