Sample requirements for proteins.

PLEASE NOTE:  The amount indicated refers to FILTRATED sample injected onto the column.
CAUTION:  Please note that all samples are filtered through a 0.22 µm filter (low binding Durapore® membrane, Millipore, Cat. No. UFC30GVNB) immediately prior to analysis.  We urge that a trial filtration is performed before sample submission to avoid/account for sample loss during this procedure.

 

Column
Optimal amount of protein
Total volume of the eluting peak
for expected 
MW>40 kDa
for expected MW
10 kDa to 40 kDa
for expected 
MW<10 kDa
Superose 6
(Pharmacia)
100 micrograms
not applicable
not applicable
~ 2mL
Superdex 200
(Pharmacia)
100 micrograms
200 - 300 micrograms
not applicable
~ 2mL
Superdex 75
(Pharmacia)
50  micrograms
100 - 200 micrograms
400 micrograms
~ 1mL

For analyses of samples that recover with less than 50% recovery from Superdex column. a $200 charge for column’s cleaning is applied

 Introduction to Light  Scattering
 Light Scattering  Theory 
 Light Scattering  Data Set 
Results for Standard Proteins
Light Scattering  Service
 Keck's Facility 
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