Determination
of molar masses for <10 mg
of protein.
The solid lines indicate the trace from the RI detector while "dots"
are weight average MW for each slice, i.e., measured every 0.5 s.
A total of ~7 mg of each protein was subjected
for analysis
(column: TSK GEL G3000SWXL [TosoHaas], buffer: 20 mM phosphate,
150 mM NaCl, pH=7.5).
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