CytoPlex—Multiplex-Core Facility

Cytokine Detection and Quantification

Cytokines are important cell signaling proteins, mediating a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are also associated with a spectrum of diseases ranging from tumor growth to infections to Parkinson’s disease. Cytokines are typically measured either by bioassay or immunoassay. Both techniques are time consuming and can facilitate the analysis of only a single cytokine at a time. For analysis of multiple cytokines, traditional methods can be time consuming, may require large sample volumes and can be very expensive.

Multiplex System for a better and easier Detection—No more ELISAs!

A new multiplex assay for the analysis of multiple proteins in a single well has been pre-tested in our laboratory and is now being offered as a service core. This suspension array system uses multiplexing technology that may one day include up to 100 color-coded bead sets, each of which can be conjugated with a different specific reactant. Each reactant is specific for a different antibody. These reactants are used to create a capture sandwich immunoassay the same as in ELISA assays. The constituents of each well are drawn up into the flow-based Bio-Plex array reader, which identifies each specific reaction based on bead color and can simultaneously quantitate up to all of the protein targets in sera, automatically analyzes samples and increases the amount of useful data per sample.

What we offer:
Cytokine panels:

Human: We are offering a 17-plex Human Cytokine panel including IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, INF gamma, MCP-1, MIP-1 beta, TNF-alpha and a cytokine mouse 10-plex including.

Mouse: We are offering a 10-plex mouse Cytokine panel including IL-1alpha, IL-3, IL-6, IL-12 (p40), IL-12 (p70), IL-17, KC, MIP-1alpha, RANTES and GCSF

Samples:
Origin: Cytokines can be evaluated in sera, cell supernatant or cell lysate.
Volume: Only a small sample volume is required. 40ul is required for serum, cell supernatant, or cell lysate.

Cost: 
Regular price: $50.00/sample
Introductory price for the Yale Community: $45.00/sample

Instructions:
Print out Cytokine Service Order Form. (PDF)

When your samples are ready, fill out the Order form, include it with your samples and ship overnight on dry ice or personally deliver it at FMB 301.

Information:

• For further information and orders please please contact Irene Visintin at: Irene.visintin@yale.edu