Galán Lab Movies
This page contains Quicktime versions of videos recorded
during the course of our research. They show different aspects of
the interaction of Salmonella with host cells. These videos are
linked to our publication. Please feel free to download them for
your personal use, but we ask that you do not distribute them or
display them for public use. If you are interested in obtaining
copies of these videos for educational purposes, please contact
us.
Note: These movies are quite large. Download time may
be very long, depending on the speed of your connection.
6.9 Mb Quicktime Movie. with cultured intestinal Henle-407 cells results in the induction
of profound cytoskeletal rearrangements that ultimately lead to
membrane ruffling and macropinocytosis.
25 Mb Quicktime Movie. (For better effect use 3D glasses).
6.8 Mb Quicktime Movie. Salmonella spp. are cytotoxic
for cultured macrophages. The cytotoxicity is initially manifested
by the inhibition of the membrane ruffling and macropinocytic activities
of the infected macrophages and is subsequently followed by cell
death. Macrophages killed by Salmonella spp. exhibit features
of apoptosis such as condensation and fragmentation of the chromatin,
membrane blebbing, the presence of cytoplasmic nucleosomes, and
apoptotic bodies. Cytotoxicity does not require bacterial internalization
since cytochalasin D, a drug that prevents bacterial uptake, does
not prevent Salmonella-induced macrophage cell death. However,
the cytotoxic effects are strictly dependent on the expression of
the invasion-associated Type III protein secretion system encoded
at centisome 63 of the Salmonella chromosome.
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Interaction
of Salmonella with cultured intestinal epithelial cells
