Recent Publications


2007
T. Ravid and M. Hochstrasser (2007). Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Nature Cell Biol., (published on-line Feb. 21, 2007; doi:10.1038/ncb1558).
2006
A.Y. Amerik, N. Sindhi, and M. Hochstrasser (2006). A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function.  J. Cell Biol. 175, 825-835.
M. Deng and M. Hochstrasser (2006). Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase. Nature 443, 827- 831.
M.F. Arteaga, L. Wang, T. Ravid, M. Hochstrasser, and C.M. Canessa (2006). A short amphipathic helix targets Sgk1 to the ER- associated ubiquitin-conjugation machinery. Proc. Natl. Acad. Sci. U.S.A. 103, 11178-11183.
O. Kerscher*, R. Felberbaum*, and M. Hochstrasser (2006). Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu. Rev. Cell Dev. Biol. 22, 159-180. [*equal contribution]
M. Hochstrasser (2006). The ubiquitin system: Key insights from Saccharomyces cerevisiae. In:  Landmark Papers in Yeast Biology.
P. Linder, D. Shore, and M.N. Hall  (eds.)  Cold Spring Harbor Laboratory Press: Cold Spring Harbor, pp. 267-284.
T. Ravid, S.G. Kreft, and M. Hochstrasser (2006). Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by
distinct pathways. EMBO J. 25, 533-543.
M. Hochstrasser (2006). Biochemical functions of ubiquitin and ubiquitin-like protein conjugation.  In:  Protein Degradation, vol. 2:   The Ubiquitin-Proteasome System. R.J. Mayer, A. Ciechanover, and M. Rechsteiner (Eds.)  Wiley-VCH Verlag: Weinheim, pp. 249-278.
M. Hochstrasser (2006). Lingering mysteries of ubiquitin-chain assembly. Cell 124, 27-34.
S.G. Kreft, L. Wang, and M. Hochstrasser (2006). Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison to its human ortholog TEB4 (MARCH VI). J. Biol. Chem. 281, 4646-4653.
J.D. Laney E.F. Mobley, and M. Hochstrasser (2006). The short-lived Matalpha2 transcriptional repressor is protected from degradation in vivo by interactions with the Tup1 and Ssn6 co-repressors. Mol. Cell. Biol. 26, 371-380.
2005
S.-J. Li, W. Hankey, and M. Hochstrasser (2005). Preparation and characterization of yeast and human desumoylating enzymes. Meth. Enzymol. 398, 457-467.
S. Huang, H. Zhou, D. Katzmann, M. Hochstrasser, E. Atanasova, and Z. Zhang (2005). Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc. Natl. Acad. Sci. U.S.A. 102, 13410-13415.
J.T. Hannich, A. Lewis, M. Kroetz, S.-J. Li, A. Emili and M. Hochstrasser (2005). Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J. Biol. Chem. 280, 4102-4110.
2004
M. Funakoshi*, X. Li*, I. Velichutina*, M. Hochstrasser and H. Kobayashi (2004). Sem1, the yeast ortholog of a human BRCA2-
binding protein, is a component of the proteasome regulatory particle and is required for its stability. J. Cell Science 117, 6447-
6457. [*equal contribution]
A.Y. Amerik and M. Hochstrasser (2004). Mechanism and function of deubiquitinating enzymes. Biochim. Biophys. Acta. [Special edition on ubiquitin]. 1695, 189-207.
J.D. Laney and M. Hochstrasser (2004). Ubiquitin-dependent control of development in Saccharomyces cerevisiae. Curr. Opin. Microbiol. 7, 647-654.
T. Ravid and M. Hochstrasser (2004). NFkappaB signaling: Flipping the switch with polyubiquitin chains. Curr. Biol. 14, R898- 900.
G. Huyer, W. F. Piluek, Z. Fansler, S.G. Kreft, M. Hochstrasser, J.L. Brodsky, and S. Michaelis (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble lumenal protein. J. Biol. Chem. 279, 38369-38378.
A.Y. Amerik and M. Hochstrasser (2004). The Saccharomyces cerevisiae deubiquitinating enzyme Doa4.  In:  Handbook of
Proteolytic Enzymes. A.J. Barrett, N.D. Rawlings and J.F. Woessner (eds.) Academic Press: New York.  Vol. 2, pp. 1229-1232.
M. Hochstrasser (2004). Ubiquitin signaling: What's in a chain? Nature Cell Biol. 6, 571-572.
I. Velichutina*, P.L. Connerly*, C.S. Arendt, X. Li, and M. Hochstrasser (2004) Plasticity in eukaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast. EMBO J. 23, 500-510. [*equal contribution]
2003
M. Hochstrasser (2003). The ubiquitin-proteasome system.  In:  Handbook of Cell Signaling.  R.A. Bradshaw and E.A. Dennis (Eds.)  Elsevier Press: San Diego. Vol. 2, pp. 347-350.
J.D. Laney and M. Hochstrasser (2003). Ubiquitin-dependent degradation of the yeast Matalpha2 transcriptional repressor enables a switch in developmental state. Genes & Devel. 17, 2259-2270.
D. Schwartz and M. Hochstrasser (2003). A superfamily of protein tags: Ubiquitin, SUMO, and related modifiers. Trends Biochem. Sciences 28, 321-328.
S.-J. Li and M. Hochstrasser (2003). The Ulp1 SUMO isopeptidase:  Distinct domains required for viability, nuclear envelope localization, and substrate specificity. J. Cell Biology 160, 1069-1081.
2002
J.D. Laney and M. Hochstrasser (2002). Assaying protein ubiquitination.  In:  Current Protocols in Protein Science, John Wiley & Sons: Rockville, MD, pp. 14.5.1-14.5.11.
M. Hochstrasser (2002) New proteases in a ubiquitin stew. Science 298, 549-552.
J.D. Laney, and M. Hochstrasser (2002) Assaying protein ubiquitination in S. cerevisiae. Meth. Enzymol. 351, 248-257.
M. Hochstrasser (2002). New structural clues to substrate specificity in the ubiquitin system. Molecular Cell 9, 453-454.
2001
R. Swanson, M. Locher and M. Hochstrasser (2001) A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum
that functions in both ER-associated and Matalpha2 repressor degradation. Genes & Devel. 15, 2660-2674.
M. Hochstrasser (2001) SP-RING for SUMO: New functions bloom for a ubiquitin-like protein. Cell 107, 5-8.
M. Hochstrasser and J. Wang (2001). Unraveling the means to the end in ATP-dependent proteases. Nature Struct. Biol. 8, 294- 296.
2000
A.Y. Amerik, S.-J. Li, and M. Hochstrasser (2000). Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae. Biol. Chem. 381, 981-992.
A.Y. Amerik, J. Nowak, S. Swaminathan, and M. Hochstrasser (2000). The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar-protein-sorting and endocytic pathways. Mol. Biol. Cell 11, 3365-3380.
M. Hochstrasser (2000). All in the ubiquitin family. Science 289, 563-564.
M. Hochstrasser (2000). Evolution and function of ubiquitin-like protein conjugation systems. Nature Cell Biol. 2, E153-E157.
R. Swanson and M. Hochstrasser (2000). A conditional ubiquitin-activating enzyme mutant for evaluating ubiquitin system function in Saccharomyces cerevisiae. FEBS Lett. 477, 193-198.
S.-J. Li and M. Hochstrasser (2000). The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein. Mol. Cell. Biol. 20, 2367-2377.