Recent Publications

 

 

2011

PDF R.J. Tomko Jr and M. Hochstrasser (2011) Incorporation of the Rpn12 subunit couples completion of proteasome regulatory particle lid assembly to lid-base joining. Mol. Cell. 44, 907-917.
PDF J.D. Laney and M. Hochstrasser (2011) Analysis of protein ubiquitination. In: Curr. Protoc. Protein Sci. John Wiley and Sons: Hoboken, NJ. Unit 14.5:pp 14.5.1-13.
PDF R. Felberbaum, N.R. Wilson, D. Cheng, J. Peng and M. Hochstrasser (2011) Desumoylation of the endoplasmic reticulum membrane VAP family protein Scs2 by Ulp1 and SUMO regulation of the inositol synthesis pathway. Mol. Cell. Biol. 32, 64-75.
PDF A.R. Kusmierczyk, M.J. Kunjappu, R.Y. Kim and M. Hochstrasser (2011) A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Nat. Struct. Mol. Biol. 18, 622-629.
PDF S.G. Kreft and M. Hochstrasser (2011) An unusual transmembrane helix in the endoplasmic reticulum ubiquitin ligase Doa10 modulates degradation of its cognate E2 enzyme. J. Biol. Chem. 286, 20163-20174.
PDF R.J. Tomko Jr and M. Hochstrasser (2011) Order of the proteasomal ATPases and eukaryotic proteasome assembly. Cell. Biochem. Biophys., 60, 13-20.
  2010

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E.M. Rubenstein and M. Hochstrasser (2010) Redundancy and variation in the ubiquitin-mediated proteolytic targeting of a transcription factor. Cell Cycle, 9, 4282-4285.

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D. Su and M. Hochstrasser (2010) A WLM Protein with SUMO-Directed Protease Activity. Mol Cell Biol., 30, 3734-3736.

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R.J. Tomko Jr, M. Funakoshi, K. Schneider, J. Wang M. Hochstrasser (2010) Heterohexameric Ring Arrangement of the Eukaryotic Proteasomal ATPases: Implications for Proteasome Structure and Assembly. Mol. Cell, 38, 393-403.

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Y. Xie, E.M. Rubenstein, T. Matt and M. Hochstrasser (2010) SUMO-independent in vivo activity of a SUMO-targeted ubiquitin ligase toward a short-lived transcription factor. Genes and Development, 24:893-903.

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A.L. Cortajarena, T.Y. Liu, M. Hochstrasser and L. Regan (2010) Designed proteins to modulate cellular networks. ACS Chem. Biol., 5(6):545-552.

 

2009

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P. Xu, D.M. Duong, N.T. Seyfried, D. Cheng, Y. Xie, J. Robert, J. Rush, M. Hochstrasser, D. Finley, and J Peng. (2009) Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell, 137(1):133-145.

 

M. Hochstrasser (2009) The ubiquitin-proteasome system. In: Handbook of Cell Signaling, 2nd edition. R.A. Bradshaw and E.A. Dennis (Eds.) Elsevier Press: San Diego. Chapter 162: pp. 1291-1294.

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M. Hochstrasser (2009) Introduction to Intracellular Protein Degradation. Chem. Rev., 109(4):1479-1480.

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C.E. Cook, M. Hochstrasser and O. Kerscher (2009) The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks. Cell Cycle, 8(7):1080-1089.

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P.A. Osmulski, M. Hochstrasser, and M. Gaczynska (2009) A Tetrahedral Transition State at the Active Sites of the 20S Proteasome Is Coupled to Opening of the alpha-Ring Channel. Structure, 17(8):1137-1147.

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M. Funakoshi, R.J. Tomko Jr. H. Kobayashi, and M. Hochstrasser (2009) Multiple Assembly Chaperones Govern Biogenesis of the Proteasome Regulatory Particle Base. Cell, 137(5):887-899.

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Hochstrasser (2009) Origin and function of ubiquitin-like proteins. Nature, 458(7237):422-9.

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M. Funakoshi and M. Hochstrasser (2009) Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Yeast, 26(3):185-192.

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M.B. Kroetz, D. Su, and M. Hochstrasser (2009) Essential Role of Nuclear Localization for Yeast Ulp2 SUMO Protease Function. Mol. Biol. Cell., 20(8):2196-2206.

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M.B. Kroetz and M. Hochstrasser (2009) Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis. Methods Mol. Biol., 497:107-120.

 

2008

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L. Wang, H. Dong, C.J. Soroka, N. Wei, J.L. Boyer, and M. Hochstrasser (2008) Degradation of the Bile Salt Export Pump at Endoplasmic Reticulum in Progressive Familial Intrahepatic Cholestasis Type II. Hepatology, 48(5):1558-1569.

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A.R. Kusmierczyk and M. Hochstrasser (2008) Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis. Biol. Chem. 389(9), 1143-1151.

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R. Felberbaum and M. Hochstrasser (2008). Ulp2 and the DNA damage response: Desumoylation enables safe passage through mitosis. Cell Cycle 7, 52-56.

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A.R. Kusmierczyk, M.J. Kunjappu, M. Funakoshi, and M. Hochstrasser (2008). A multimeric assembly factor controls formation of alternative 20S proteasomes. Nat. Struct. Mol. Biol. 15, 237-244.

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T. Ravid and M. Hochstrasser (2008). Diversity of degradation signals in the ubiquitin–proteasome system. Nat. Rev. Mol. Cell Biol. 9(9), 679-690.

 

M. Hochstrasser, M. Deng, A.R. Kusmierczyk, X. Li, S. Kreft, T. Ravid, M. Funakoshi, M. Kunjappu and Y. Xie (2008). Molecular genetics of the ubiquitin-proteasome system: Lessons from yeast. In: Ernst Schering Foundation Symposia Proceedings (Springer Verlag), pp. 41-66.

 

2007

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T. Ravid and M. Hochstrasser (2007). Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Nature Cell Biol., 9(4), 422-427.

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X. Li, A.R. Kusmierczyk, P. Wong, A. Emili, and M. Hochstrasser (2007). beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J. 26, 2339-2349.

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D.C. Schwartz, R. Felberbaum, and M. Hochstrasser (2007). The Ulp2 SUMO protease is required for cell division following termination of the DNA damage checkpoint. Mol. Cell. Biol. 27, 6948-6961.

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A. Lewis*, R. Felberbaum*, and M. Hochstrasser (2007). A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance. J. Cell Biol. 178, 813-827. [*equal contribution]

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Y. Xie*, O. Kerscher*, M. Kroetz, H. McConchie, P. Sung, and M. Hochstrasser (2007). The yeast Hex3-Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation. J. Biol. Chem. 282, 34176-34184. [*equal contribution]

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M. Hochstrasser (2007). Ubiquitin ligation without a ligase. Dev. Cell 13, 4-6.

 

2006

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A.Y. Amerik, N. Sindhi, and M. Hochstrasser (2006). A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function.  J. Cell Biol. 175, 825-835.

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M. Deng and M. Hochstrasser (2006). Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase. Nature 443, 827- 831.

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M.F. Arteaga, L. Wang, T. Ravid, M. Hochstrasser, and C.M. Canessa (2006). A short amphipathic helix targets Sgk1 to the ER- associated ubiquitin-conjugation machinery. Proc. Natl. Acad. Sci. U.S.A. 103, 11178-11183.

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O. Kerscher*, R. Felberbaum*, and M. Hochstrasser (2006). Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu. Rev. Cell Dev. Biol. 22, 159-180. [*equal contribution]

 

M. Hochstrasser (2006). The ubiquitin system: Key insights from Saccharomyces cerevisiae. In:  Landmark Papers in Yeast Biology.

P. Linder, D. Shore, and M.N. Hall  (eds.)  Cold Spring Harbor Laboratory Press: Cold Spring Harbor, pp. 267-284.

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T. Ravid, S.G. Kreft, and M. Hochstrasser (2006). Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by

distinct pathways. EMBO J. 25, 533-543.

 

M. Hochstrasser (2006). Biochemical functions of ubiquitin and ubiquitin-like protein conjugation.  In:  Protein Degradation, vol. 2:   The Ubiquitin-Proteasome System. R.J. Mayer, A. Ciechanover, and M. Rechsteiner (Eds.)  Wiley-VCH Verlag: Weinheim, pp. 249-278.

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M. Hochstrasser (2006). Lingering mysteries of ubiquitin-chain assembly. Cell 124, 27-34.

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S.G. Kreft, L. Wang, and M. Hochstrasser (2006). Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison to its human ortholog TEB4 (MARCH VI). J. Biol. Chem. 281, 4646-4653.

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J.D. Laney E.F. Mobley, and M. Hochstrasser (2006). The short-lived Matalpha2 transcriptional repressor is protected from degradation in vivo by interactions with the Tup1 and Ssn6 co-repressors. Mol. Cell. Biol. 26, 371-380.

 

2005

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S.-J. Li, W. Hankey, and M. Hochstrasser (2005). Preparation and characterization of yeast and human desumoylating enzymes. Meth. Enzymol. 398, 457-467.

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S. Huang, H. Zhou, D. Katzmann, M. Hochstrasser, E. Atanasova, and Z. Zhang (2005). Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc. Natl. Acad. Sci. U.S.A. 102, 13410-13415.

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J.T. Hannich, A. Lewis, M. Kroetz, S.-J. Li, A. Emili and M. Hochstrasser (2005). Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J. Biol. Chem. 280, 4102-4110.

 

2004

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M. Funakoshi*, X. Li*, I. Velichutina*, M. Hochstrasser and H. Kobayashi (2004). Sem1, the yeast ortholog of a human BRCA2-

binding protein, is a component of the proteasome regulatory particle and is required for its stability. J. Cell Science 117, 6447-

6457. [*equal contribution]

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A.Y. Amerik and M. Hochstrasser (2004). Mechanism and function of deubiquitinating enzymes. Biochim. Biophys. Acta. [Special edition on ubiquitin]. 1695, 189-207.

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J.D. Laney and M. Hochstrasser (2004). Ubiquitin-dependent control of development in Saccharomyces cerevisiae. Curr. Opin. Microbiol. 7, 647-654.

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T. Ravid and M. Hochstrasser (2004). NFkappaB signaling: Flipping the switch with polyubiquitin chains. Curr. Biol. 14, R898- 900.

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G. Huyer, W. F. Piluek, Z. Fansler, S.G. Kreft, M. Hochstrasser, J.L. Brodsky, and S. Michaelis (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble lumenal protein. J. Biol. Chem. 279, 38369-38378.

 

A.Y. Amerik and M. Hochstrasser (2004). The Saccharomyces cerevisiae deubiquitinating enzyme Doa4.  In:  Handbook of

Proteolytic Enzymes. A.J. Barrett, N.D. Rawlings and J.F. Woessner (eds.) Academic Press: New York.  Vol. 2, pp. 1229-1232.

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M. Hochstrasser (2004). Ubiquitin signaling: What's in a chain? Nature Cell Biol. 6, 571-572.

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I. Velichutina*, P.L. Connerly*, C.S. Arendt, X. Li, and M. Hochstrasser (2004) Plasticity in eukaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast. EMBO J. 23, 500-510. [*equal contribution]

 

2003

 

M. Hochstrasser (2003). The ubiquitin-proteasome system.  In:  Handbook of Cell Signaling.  R.A. Bradshaw and E.A. Dennis (Eds.)  Elsevier Press: San Diego. Vol. 2, pp. 347-350.

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J.D. Laney and M. Hochstrasser (2003). Ubiquitin-dependent degradation of the yeast Matalpha2 transcriptional repressor enables a switch in developmental state. Genes & Devel. 17, 2259-2270.

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D. Schwartz and M. Hochstrasser (2003). A superfamily of protein tags: Ubiquitin, SUMO, and related modifiers. Trends Biochem. Sciences 28, 321-328.

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S.-J. Li and M. Hochstrasser (2003). The Ulp1 SUMO isopeptidase:  Distinct domains required for viability, nuclear envelope localization, and substrate specificity. J. Cell Biology 160, 1069-1081.

 

2002

 

J.D. Laney and M. Hochstrasser (2002). Assaying protein ubiquitination.  In:  Current Protocols in Protein Science, John Wiley & Sons: Rockville, MD, pp. 14.5.1-14.5.11.

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M. Hochstrasser (2002) New proteases in a ubiquitin stew. Science 298, 549-552.

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J.D. Laney, and M. Hochstrasser (2002) Assaying protein ubiquitination in S. cerevisiae. Meth. Enzymol. 351, 248-257.

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M. Hochstrasser (2002). New structural clues to substrate specificity in the ubiquitin system. Molecular Cell 9, 453-454.

 

2001

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R. Swanson, M. Locher and M. Hochstrasser (2001) A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum

that functions in both ER-associated and Matalpha2 repressor degradation. Genes & Devel. 15, 2660-2674.

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M. Hochstrasser (2001) SP-RING for SUMO: New functions bloom for a ubiquitin-like protein. Cell 107, 5-8.

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M. Hochstrasser and J. Wang (2001). Unraveling the means to the end in ATP-dependent proteases. Nature Struct. Biol. 8, 294- 296.

 

2000

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A.Y. Amerik, S.-J. Li, and M. Hochstrasser (2000). Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae. Biol. Chem. 381, 981-992.

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A.Y. Amerik, J. Nowak, S. Swaminathan, and M. Hochstrasser (2000). The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar-protein-sorting and endocytic pathways. Mol. Biol. Cell 11, 3365-3380.

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M. Hochstrasser (2000). All in the ubiquitin family. Science 289, 563-564.

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M. Hochstrasser (2000). Evolution and function of ubiquitin-like protein conjugation systems. Nature Cell Biol. 2, E153-E157.

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R. Swanson and M. Hochstrasser (2000). A viable ubiquitin-activating enzyme mutant for evaluating ubiquitin system function in Saccharomyces cerevisiae. FEBS Lett. 477, 193-198.

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S.-J. Li and M. Hochstrasser (2000). The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein. Mol. Cell. Biol. 20, 2367-2377.