Stem Cell Preparation & Analysis

Director: Diane Krause
Associate Director: Bernard Forget

Overview

The Stem Cell Preparation & Analysis Core provides several services:

1. Instrumentation and technical expertise in performing large-scale selection and cryopreservation of human peripheral blood stem cells, and has IRB approval to provide PBSC or subfractions of PBSC in large or small quantities. Included within this resource is provision of large-scale selections of CD34 cells using any of 3 clinical scale immunomagnetic devices.

2. Small-scale isolation of murine hematopoietic populations from bone marrow and fetal liver and human cell subpopulations, as well as training in the isolation techniques used.

3. Performs for and/or trains investigators to perform, colony forming unit analyses (CFU-C) for murine and human cells.

4. Produces myeloid cell lines from primary bone marrow cells.

5. Performs small or large scale in vitro differentiation of primary hematopoietic stem and progenitor cells down the erythroid lineage.

6. Xenogeneic transplantation of human cells into immunodeficient mouse strains. Training is a major component of all of these services so that, if desired, individual investigators can perform these assays in their own laboratories and use the core as a resource for trouble shooting. For more information, contact Diane Krause, Director.