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Hematopoietic
Colony:
 Transduction
of the Beta-galactosidase gene into hematopoietic progenitor cells.
Experimental gene transfer into hematopoietic progenitor cells has
been used to successfully treat adenosine deaminase deficient severe
combined immunodeficiency as well being employed in clinical trials
for a host of malignant and non-malignant disorders. In this experiment,
hematopoietic progenitors expressing the transgene were selected by
cell sorting and plated into soft agar hematopoietic colony assays
- CFU-gemm that develop may then express gene activity, measured by
X-gal staining in this picture which causes the transduced colony
to appear blue whereas non-transduced colonies (in this case a CFU-gm)
appear white. For more information, see:
- Strair
RK, Towle M, Heald P, Smith BR. 1990. Retroviral Mediated Transfer
and Expression of Exogenous Genes in Primary Lymphoid Cells: Assaying
for a Viral Transactivator Activity in Normal and Malignant Cells.
Blood 76:1201-1208.
- Strair
RK, Towle M, Smith BR. 1990. Retroviral Mediated Gene Transfer
into Bone Marrow Progenitor Cells: Use of Beta-Galactosidase as
a Selectable Marker. Nuc Acids Res 18:4759-62.
- Ross
GS, Wilson DJ, Erikson RP, Motulsky AG, Parkman R, Samulski RJ,
Straus SE, Smith, BR. 1996. Gene Therapy in the United States:
A 5 Year Status Report. Human Gene Therapy 7:1781-1790.
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