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Diane Krause, MD, PhD
Professor of Laboratory Medicine and Cell Biology
Associate Director, Yale Stem Cell Center
Associate Director, Transfusion Medicine Service
Director, Frisbee Laboratory for Hematopoietic Graft Engineering
Director, Yale Center of Excellence in Molecular Hematology

Krause Laboratory
Amistad 237
203-737-1678
diane.krause@yale.edu

Undergraduate: ScB, 1982, Brown University
MD: 1990, University of Pennsylvania
PhD: 1990, University of Pennsylvania
Clinical Pathology Residency: University of Pennsylvania
Research Fellowship: Johns Hopkins Oncology Center

Community of Science Biosketch

Specifically, my laboratory is pursuing three projects. The first is focused on defining the molecular mechanism(s) that regulate gene expression during developmental hematopoiesis. Using molecular biological techniques, I am identifying promoter and silencer regions upstream of the CD34 gene, and defining the protein factors that regulate CD34 expression via interaction with these critical regions. In addition, we are using PCR subtraction techniques and cDNA arrays to identify additional genes that are expressed exclusively on hematopoietic stem cells. The second focus in my laboratory is the development and use of murine bone marrow transplantation models for a.) selection of stem cells, b.) purging of unwanted cells (in the autologous setting), c.) gene therapy, and d.) as a predictive model for human cell engraftment in clinical transplantation. Projects include using limiting numbers of CD34+ and CD34- bone marrow cells to engraft lethally irradiated mice, and characterizing gene expression in the confirmed stem cell populations by RT-PCR, Northern analysis, screening arrays and FACS analysis. Also, related to this focus are studies in which we use human stem cells to engraft in highly immunodeficient mice to determine the functional capacity of the human cells. The third focus in the lab is the identification of cells in the bone marrow that are capable of differentiating into nonhematopoietic tissues including chondrocytes, osteoblasts, myocytes, pneumocytes and hepatocytes.

References

1. Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis SJ. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 105:369-377, 2001.
2. Perez L, Rinder HM, Wang C, Tracey JB, Maun N, Krause DS. Xenotransplantation of immunodeficient mice with human CD34+ cells provides an in-vivo model for munam megakaryocytopoiesis and platelet production . Blood 97:1635-43, 2001.
3. Taranenko N, Krause DS. Regulation of CD34 transcription by Sp1 requires sites upstream and downstream of the transcription start site. Experimental Hematology, 28: 974-984, 2000.
4. Theise ND, Nimmakayalu M, Gardner R, Illei PB, Morgan G, Teperman L, Henegariu O, Krause DS. Liver from bone marrow in humans. Hepatology, 32: 11-16, 2000.
   
5. Theise ND, Badve S, Saxena R, Henegariu O, Sell S, Crawford JM, Krause DS. Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation. Hepatology, 31: 235-240, 2000.

For more information, please visit the Krause Laboratory.