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John Greg Howe, PhD

Assistant Professor of Laboratory Medicine
Director, Molecular Diagnostics Laboratory
FMB 540
203-737-4237
john.howe@yale.edu

B.S., 1973, California State University (Northridge)
M.A., 1975, University of California (Santa Barbara)
Ph.D., 1983, University of California (Davis)
Fellowship: Yale University

The mission of the Molecular Diagnostics Laboratory is to develop and bring into clinical use already published molecular pathology assays as well as to develop new and innovative molecular tests for the diagnosis of disease and the monitoring of therapy. Various tests have been developed and are in clinical use such as detection of mutations in the prothrombin and Factor V genes that are associated with coagulation abnormalities and tests to determine monoclonality in patients with suspected hematological malignancies. Previously, we have developed an in situ hybridization assay to test for the presence of Epstein-Barr virus (EBV) in fixed tissue and have used this test to study the role of EBV in several diseases. Using the in situ hybridization method in conjunction with flow cytometry we have been able to demonstrate the utility of a method to quantitate the number of EBV containing cells. We have applied quantitative polymerase chain reaction (QPCR) methods to the measurement of EBV viral load in allogenic transplant patients and have further refined the method with the development of a real-time quantitative EBV PCR assay.

1. Salloum E, Cooper DL, Howe G, Lacy J, Tallini G, Crouch J, Schultz M and Murren J. Spontaneous Regression of Lymphoproliferative Disorders in Patients Treated With Methotrexate for Rheumatoid Arthritis and Other Rheumatic Diseases. J Clin Oncol 14:1943-1949, 1996.
   
2. Madison, J.F., Cooper, D., Howe, G., Crotty, P.L., Tallini G., Crouch, J. and R.N. Eisen (1996). Lymphomatoid granulomatosis: A T-cell rich B-Cell lymphoproliferative disorder. Archives Dermatology 132, 1464-147.
   
3. Crouch, J., Leitenberg, D., Smith, B.R. and G. Howe (1997). Epstein-Barr Virus suspension Cell Assay using In Situ Hybridization and Flow Cytometry. Cytometry 29:50-57.
   
4. Brodie, S.C., De la Rosa, C., Howe, JG, Travis, W. D., Crouch, J., Diem, K. (1999). Pediatric AIDS-Associated Lymphocytic Interstitial Pneumonia and Pulmonary Atrerio-occlusive Disease: Role of VCAM-1/VLA-4 Adhesion Pathway and Human Herpesviruses. American Journal of Pathology 154:1453-64.