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Case Study#4

  1  2  3   4  5   6   7  8  9  10  11  12  13  14  15  16  17  foward

Identification of Mycobacteria

  • Growth rate
  • Temperature range of growth
  • Colony morphology
  • Colony pigment
  • Biochemical tests
  • Ribosomal RNA probes
  • High Performance Liquid Chromatography (HPLC)

Organism Characteristics

  • Growth in three days on Blood agar at 37°C
  • Color:
    • Tan, buff
    • No change with light exposure
  • Colony morphology: rough

    Initial impression: Rapid grower (Runyun Group IV)

Identification of Organism

  • Sent to West Haven Veteran's Administration Medical Center for HPLC - central laboratory for system
  • HPLC technique based on species-specific characteristics of mycolic acids in the cell wall

 

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Page last revised: July 8, 2008