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Case Study#4

  1  2  3   4  5   6   7  8  9  10  11  12  13  14  15  16  17  foward

HPLC

  • Extraction procedure
    • Saponification to cleave mycolic acids
    • Extraction into chloroform

    Mycolic acids converted to UV absorbing p-bromophenacyl esters

  • Interpretation of patterns
    • Late emerging single-cluster peak pattern


    • Widely separated double-peak clusters

     

  • Examples of rapid growers
    • M. fortuitum complex


    • M. chelonae complex

 

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