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Chromosomal
Gains and Losses in Testicular Germ Cell Tumors of Adolescents and Adults
Investigated by a Modified Comparative Genomic Hybridization Approach |
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Carla Rosenberg,
Tom Bakker Schut, Marijke Mostert, Hans Tanke, Anton Raap, J. Wolter Oosterhuis,
and Leendert Looijenga |
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Laboratory
of Cytochemistry and Cytometry (CR, HT, AR), Department of Molecular Cell
Biology, Leiden University Medical Center; Department of Pathology (CR,
MM, JWO, LL), Laboratory for Experimental Patho-Oncology, Josephine Nefkens
Institute, Daniel den Hoed Cancer Center, University Hospital Rotterdam;
and Department General Surgery (TBS), Erasmus University Rotterdam, Rotterdam,
The Netherlands |
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SUMMARY: Testicular germ
cell tumors of adolescents and adults, both seminomas (SE) and nonseminomas
(NS), are aneuploid, and classical karyotyping demonstrated a specific pattern
of gains and losses. More recently, these data have been supported by in
situ hybridization and comparative genomic hybridization (CGH) on a limited
number of samples. Interpretation of CGH results is complicated by the intermediate
ploidy of these tumors (3-4 n for SE and 2-3 n for NS). To
circumvent this problem, this particular study was undertaken. CGH was performed
on 8 SE and 10 NS, after which two single chromosome normalizations were
applied, one for chromosome 4 (found to be associated to the lower ploidy
level of the tumor) and one for chromosome 8 (found to be associated with
the higher ploidy level of the tumor). Using this modified CGH interpretation
method, chromosomal regions with a similar copy number of chromosome 4 and
8 were identified as belonging to the lower and higher ploidy level, and
the regions below chromosome 4 and above chromosome 8 were identified as
lost or gained outside the ploidy range of the tumor, respectively. Our
results are in accordance with earlier findings, however they add novel
data, including comparison of SE and NS. This approach reveals relevant
information about the chromosomal constitution of testicular germ cell tumors,
leading to a better understanding of the pathogenesis of these tumors.
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