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Pleomorphic
Adenoma Gene 1 is Expressed in Cultured Benign and Malignant Salivary Gland
Tumor Cells |
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Lurdes Queimado,
Carla Lopes, Fenghe Du, Carmo Martins, Anne M. Bowcock, Jorge Soares, and
Michael Lovett |
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Departments
of Patologia Morfológica and Centro de Investigação
de Patobiologia Molecular (LQ, CL, CM, JS), Instituto Português de
Oncologia de Francisco Gentil, Lisboa, Portugal; and The McDermott Center
(LQ, CL, FD, AMB, ML), University of Texas Southwestern Medical Center,
Dallas, Texas |
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The pleomorphic adenoma
gene 1 (PLAG1) is activated by reciprocal chromosomal translocations
involving 8q12 in a subset of salivary gland pleomorphic adenomas. PLAG1
encodes a zinc finger protein and was initially reported to be expressed
in placenta and fetal tissues, with no detectable expression in other normal
adult tissues. By Northern blotting we have detected PLAG1 expression
in a wide set of normal adult tissues, including heart, placenta, spleen,
prostate, testis, ovary, and small intestine. We have performed reverse
transcriptase-PCR and Northern blot analyses to study the expression of
PLAG1 in normal salivary gland tissues and in primary cultures and
cell lines derived from salivary gland tumors. PLAG1 was expressed
in all tumor-derived primary cultures and cell lines, irrespective of their
histological type or the presence of genomic rearrangements involving PLAG1,
but was not detected by our assays in normal salivary glands. Our data indicate
that the presence or absence of PLAG1 expression is not an unequivocal
marker for the differential diagnosis of benign versus malignant salivary
gland tumors, and that a simple de novo activation of this gene does not
fully explain the involvement of this gene in salivary gland tumors. (Lab
Invest, 79:583-589). |
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