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Reduced
Expression of Calponin h1 in Leiomyosarcoma of the Uterus |
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Akiko Horiuchi,
Toshio Nikaido, Kazuko Ito, Ya-li Zhai, Ayaka Orii, Shunichiro Taniguchi,
Toshihiko Toki, and Shingo Fujii |
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Departments
of Obstetrics and Gynecology (AH, TN, KI, Y-LZ, AO, TT) and Molecular Oncology
and Angiology (ST), Shinshu University School of Medicine, Matsumoto; and
Department of Gynecology and Obstetrics (SF), Faculty of Medicine, Kyoto
University, Kyoto, Japan |
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To determine the genetic
differences between various smooth muscle tumors of the uterus, the expression
of calponin h1 (cytoskeletal protein) was examined in normal myometrium
and in smooth muscle tumors of the uterus (leiomyosarcoma, smooth muscle
tumors of uncertain malignant potential, cellular leiomyoma, bizarre leiomyoma,
and ordinary leiomyoma) using immunohistochemistry and Western blotting.
Analysis of calponin h1 transcripts using the reverse-transcription-PCR
was also performed. Immunohistochemically, calponin h1 was expressed in
normal myometrium and in all leiomyomas; however, its expression was markedly
weaker in leiomyosarcoma. The results of both Western blotting and the analysis
of calponin h1 transcripts were compatible with the immunohistochemical
results. It is suggested that reduced expression of calponin h1 is associated
with leiomyosarcoma of the uterus, and that calponin h1 expression may serve
as a valuable molecular maker in the differential diagnosis of smooth muscle
tumors of the uterus. |
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