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Trisomies of chromosomes
3q, 7, 8, 12, 16, 17q, and 20 and the loss of the Y chromosome are specific
genetic changes in papillary renal cell tumors. Many papillary renal cell
tumors show marker chromosomes by karyotyping, which may contain duplicated
chromosomal sequences. To uncover such alterations, we have analyzed 35
papillary renal cell tumors for each chromosome arm mentioned above and
also for the X and Y chromosomes by employing a fluorescent microsatellite
assay. We detected allelic duplications at the following chromosomal regions:
7q31-33 (64%), 17q12-22 (70%), 16q24-qter (55%), 12q12-14 (42%), 8p21 (25%),
3q22-24 (24%), and 20q13 (48%). The Y chromosome was missing in 74% of tumors
obtained from male patients. No deletion at chromosome 3p was detected.
The microsatellite assay revealed several allelic duplications at the specific
chromosomal regions in papillary renal cell tumors, which either showed
rearranged chromosomes of unknown origin or did not show specific alterations
by previous karyotyping. |
