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Absence
of Mast Cell Involvement in Leukocyte Adhesion and Emigration Induced by
Inhibition of Nitric Oxide Synthase |
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Hiroaki Kosaka,
Akitoshi Seiyama, Nobuyuki Terada, Hirohito Yoneyama, Seiichi Hirota, and
Yukihiko Kitamura |
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Second
Department of Physiology (HK, HY), Kagawa Medical University, Kita, Kagawa;
Departments of Physiology (AS, NT) and Pathology (SH, YK), Osaka University
Medical School, Suita, Osaka; and Department of Pathology (NT), Hyogo College
of Medicine, Nishinomiya, Hyogo, Japan |
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Leukocyte adhesion on venules
and their emigration to extravascular connective tissue are induced by administration
of a nitric oxide synthase (NOS) inhibitor NG-nitro-l-arginine methyl ester.
In the present study, the involvement of mast cells in the process was examined
in genetically mast cell-deficient and normal rats by intravital microscopy.
Superfusion of the NOS inhibitor into the mesentery induced partial degranulation
of mast cells in normal rats. However, leukocyte adhesion on mesenteric
venules and emigration to extravascular connective tissue occurred even
in mast cell-deficient rats, with no significant difference from the normal
rats. When the reverse-passive Arthus reaction, characterized by generalized
antigen-antibody complex formation, was induced in the rat mesentery, the
immune complex increased both the adhesion and emigration in normal rats
but not in mast cell-deficient rats. These results show that mast cells
are not involved in the leukocyte adhesion and emigration induced by NOS
inhibition, but are in the reverse-passive Arthus reaction. |
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