Fluorochromes and Capabilities
| Instruments | Laser | Excitation Laser Line (nm) | Fluorescence Channel | Fluorochromes | ||||||
| BD FACScan | Argon (L1) | 488 | FL1 Green | FITC | Alexa Fluor 488 | |||||
| FL2 Yellow | PE | PI | EMA | |||||||
| FL3 Red | PE-Texas Red | PE-Cy5 | PerCP | PerCP-Cy5.5 | PE-Cy7 | |||||
| BD FACSCalibur | Argon (L1) | 488 | FL1 Green | FITC | Alexa Fluor 488 | |||||
| FL2 Yellow | PE | |||||||||
| FL3 Red | PE-Texas Red | PE-Cy5 | PerCP | PerCP-Cy5.5 | PE-Cy7 | |||||
| Red Diode (L2) | 635 | FL4 Red | APC | Alexa Fluor 647 | ||||||
| BD FACSVantage | Argon (L1) | 488 | FL1 Green | FITC | Alexa Fluor 488 | |||||
| FL2 Yellow | PE | PI | EMA | |||||||
| FL3 Red | PE-Texas Red | PE-Cy5 | PerCP | PerCP-Cy5.5 | PE-Cy7 | |||||
| (L3) | 568 | RFP | Texas-Red | Alexa 568 | ||||||
| HeNe (L2) | 633 | FL4 Red | APC | Alexa Fluor 647 | Alexa Fluor 680 | Alexa Fluor 700 | ||||
| FL5 InfraRed | APC-Cy7 | |||||||||
| BD FACSAria | Argon (L1) | 488 | Green | FITC | Alexa Fluor 488 | |||||
| Yellow | PE | |||||||||
| Red | PE-Texas Red | PE-Cy5 | PerCP | PI | EMA | |||||
| Far Red | PerCP-Cy5.5 | |||||||||
| Infra Red | PE-Cy7 | |||||||||
| HeNe (L2) | 633 | Red | APC | Alexa Fluor 647 | Alexa Fluor 680 | Alexa Fluor 700 | ||||
| Infra Red | APC-Cy7 | |||||||||
| Violet (L3) | 407 | Blue | Alexa Fluor 405 | Pacific Blue | ||||||
| BD LSR II | Argon (L1) | 488 | Green | FITC | Alexa Fluor 488 | |||||
| Yellow | PE | |||||||||
| Red | PE-Texas Red | PE-Cy5 | PerCP | PI | EMA | |||||
| Far Red | PerCP-Cy5.5 | |||||||||
| Infra Red | PE-Cy7 | |||||||||
| HeNe (L2) | 633 | FL4 Red | APC | Alexa Fluor 647 | Alexa Fluor 680 | Alexa Fluor 700 | ||||
| Infra Red | APC-Cy7 | |||||||||
| UV (L3) | 355 | Violet | ||||||||
| Blue | Alexa Fluor 405 | Pacific Blue | ||||||||
| Violet (L4) | 407 | Blue | Alexa Fluor 405 | Pacific Blue | ||||||
| BD LSR II Green | Argon (L1) | 488 | Green | FITC | Alexa Fluor 488 | |||||
| Yellow | PE | |||||||||
| Red | PE-Texas Red | PE-Cy5 | PerCP | PI | EMA | |||||
| Far Red | PerCP-Cy5.5 | |||||||||
| Infra Red | PE-Cy7 | |||||||||
| HeNe (L2) | 633 | FL4 Red | APC | Alexa Fluor 647 | Alexa Fluor 680 | Alexa Fluor 700 | ||||
| Infra Red | APC-Cy7 | |||||||||
| L3 | 532 | Green | CMTMR | Alexa Fluor 532 | Alexa Fluor 568 | |||||
| Blue | Alexa Fluor 405 | Pacific Blue | ||||||||
| Violet (L4) | 407 | Blue | Alexa Fluor 405 | Pacific Blue | ||||||
| *Data from the chart above may be found at | ||||||||||
| www.bdbiosciences.com | ||||||||||
| Dako MoFlo | Argon (L1) | 488 | Green | FITC | Alexa Fluor 488 | |||||
| Yellow | PE | PI | EMA | |||||||
| Red | PE-Texas Red | PE-Cy5 | PerCP | |||||||
| Far Red | PerCP-Cy5.5 | |||||||||
| Infra Red | PE-Cy7 | |||||||||
| HeNe (L2) | 633 | FL5,6,7 Red | APC | Alexa Fluor 647 | Alexa Fluor 680 | Alexa Fluor 700 | ||||
| Infra Red | APC-Cy7 | |||||||||