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FACS Aria* |
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Violet Laser - 407nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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| |
A |
530/30 |
502LP |
Alexa Fluor 430, CFP |
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B |
450/40 |
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Cascade Blue, Pacific Blue, DAPI,
Hoechst, Alexa Fluor 405, BFP, Marina Blue |
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Blue Laser - 488nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
780/60 |
735LP |
PE-Cy7, PE-Alexa Fluor 750 |
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B |
695/40 |
655LP |
PI (with PE), PerCP, PerCP-Cy5.5,
PE-Cy5, EMA |
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C |
610/20 |
595LP |
PE-Texas Red, PI (with PE and PE-Cy5.5) |
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D |
576/26 |
556LP |
PE, PI (with PerCP) |
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E |
530/30 |
502LP |
FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14 |
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F |
488/10 |
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SSC |
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Red Laser - 633nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
780/60 |
735LP |
APC-Cy7, APC-Alexa 750 |
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B |
710/25 |
685LP |
Alexa Fluor 680, Alexa Fluor 700 |
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C |
660/20 |
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APC, Alexa Fluor 647 |
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*Please
consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to |
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determine which
fluorochromes cannot be used together due to high spectral overlaps. |
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LSRII* |
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UV Laser - 355nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
530/30 |
450LP |
Indo-1 (Blue), Hoechst Red, Fura-2,
Am-Cyan |
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B |
440/40 |
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Indo-1 (Violet), DAPI, Alexa Fluor
350, Hoechst |
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Violet Laser - 405nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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| |
A |
525/50 |
505LP |
Alexa Fluor 430, CFP, Am Cyan |
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B |
440/40 |
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Cascade Blue, Pacific Blue, DAPI,
Hoechst, Alexa Fluor 405, BFP, Marina Blue |
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Blue Laser - 488nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
780/60 |
735LP |
PE-Cy7, Alexa 750 PE |
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B |
670/14 |
635LP |
PI (with PE), PerCP, PerCP-Cy5.5,
PE-Cy5, EMA |
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C |
610/20 |
600LP |
PE-Texas Red, PI (with PE and PE-Cy5.5) |
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D |
576/26 |
550LP |
PE, PI (with PerCP), Pynonin |
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E |
530/30 |
505LP |
FITC, GFP, CFSE, YFP, Alexa Fluor 488 |
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F |
488/10 |
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SSC |
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Red Laser - 633nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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| |
A |
780/60 |
735LP |
APC-Cy7, APC-Alexa Fluor 750 |
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B |
710/25 |
685LP |
Alexa Fluor 680, Alexa Fluor 700 |
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C |
660/20 |
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APC, Alexa Fluor 647 |
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*Please
consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to |
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| |
determine which
fluorochromes cannot be used together due to high spectral overlaps. |
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LSRII Green* |
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Violet Laser - 407nm |
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| |
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| |
PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
525/50 |
505LP |
Alexa Fluor 430, CFP, Am Cyan |
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B |
450/50 |
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Cascad Blue, Pacific Blue, DAPI,
Hoechst, Alexa Fluor 405, BFP, CFP |
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Blue Laser - 488nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
670/14 |
635LP |
PerCP |
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B |
525/50 |
505LP |
FITC, GFP, PKH67, CFSE, Alexa Fluor 488 |
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C |
488/10 |
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SSC |
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Green Laser - 532nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
780/60 |
735LP |
PE-Cy7, |
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B |
710/50 |
685Lp |
PI,
PE-Cy5.5, |
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C |
660/20 |
630LP |
PE-Cy5 |
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D |
610/20 |
600LP |
Alexa Fluor 594, PE-Texas Red, Alexa
Fluor 568, PI, Rhodamine-B, PI (with PE and
PE-Cy5.5) |
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E |
575/26 |
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PE, DsRed, RFP, Pyronin-Y,
PKH26, |
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Red Laser - 633nm |
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PMT |
Bandpass |
Long Pass |
Fluorochrome |
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A |
780/60 |
755LP |
APC-Cy7, APC-Alexa Fluor 750 |
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B |
710/50 |
690LP |
Alexa Fluor 680, Alexa Fluor 700 |
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C |
660/20 |
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APC, Alexa Fluor 647 |
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| |
|
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| |
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| |
*Please
consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to |
|
|
| |
determine which
fluorochromes cannot be used together due to high spectral overlaps. |
|
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