FACS Aria*  
   
  Violet Laser - 407nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 530/30 502LP Alexa Fluor 430, CFP  
  B 450/40   Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue  
   
  Blue Laser - 488nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 735LP PE-Cy7, PE-Alexa Fluor 750  
  B 695/40 655LP PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA  
  C 610/20 595LP PE-Texas Red, PI (with PE and PE-Cy5.5)  
  D 576/26 556LP PE, PI (with PerCP)  
  E 530/30 502LP FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14  
  F 488/10   SSC  
   
  Red Laser - 633nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 735LP APC-Cy7, APC-Alexa 750  
  B 710/25 685LP Alexa Fluor 680, Alexa Fluor 700  
  C 660/20   APC, Alexa Fluor 647  
   
   
  *Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to   
  determine which fluorochromes cannot be used together due to high spectral overlaps.  
           
  LSRII*  
   
  UV Laser - 355nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 530/30 450LP Indo-1 (Blue), Hoechst Red, Fura-2, Am-Cyan  
  B 440/40   Indo-1 (Violet), DAPI, Alexa Fluor 350, Hoechst  
   
  Violet Laser - 405nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 525/50 505LP Alexa Fluor 430, CFP, Am Cyan  
  B 440/40   Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue  
   
  Blue Laser - 488nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 735LP PE-Cy7, Alexa 750 PE  
  B 670/14 635LP PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA  
  C 610/20 600LP PE-Texas Red, PI (with PE and PE-Cy5.5)  
  D 576/26 550LP PE, PI (with PerCP), Pynonin  
  E 530/30 505LP FITC, GFP, CFSE, YFP, Alexa Fluor 488  
  F 488/10   SSC  
   
  Red Laser - 633nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 735LP APC-Cy7, APC-Alexa Fluor 750  
  B 710/25 685LP Alexa Fluor 680, Alexa Fluor 700  
  C 660/20   APC, Alexa Fluor 647  
   
   
  *Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to   
  determine which fluorochromes cannot be used together due to high spectral overlaps.  
           
  LSRII Green*  
   
  Violet Laser - 407nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 525/50 505LP Alexa Fluor 430, CFP, Am Cyan  
  B 450/50   Cascad Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, CFP  
   
  Blue Laser - 488nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 670/14 635LP PerCP  
  B 525/50 505LP FITC, GFP, PKH67, CFSE, Alexa Fluor 488  
  C 488/10   SSC  
   
  Green Laser - 532nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 735LP PE-Cy7,  
  B 710/50 685Lp PI, PE-Cy5.5,   
  C 660/20 630LP PE-Cy5  
  D 610/20 600LP Alexa Fluor 594, PE-Texas Red, Alexa Fluor 568, PI, Rhodamine-B, PI (with PE and PE-Cy5.5)  
  E 575/26   PE, DsRed, RFP, Pyronin-Y, PKH26,   
   
  Red Laser - 633nm  
   
  PMT Bandpass Long Pass Fluorochrome  
  A 780/60 755LP APC-Cy7, APC-Alexa Fluor 750  
  B 710/50 690LP Alexa Fluor 680, Alexa Fluor 700  
  C 660/20   APC, Alexa Fluor 647  
   
   
  *Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to   
  determine which fluorochromes cannot be used together due to high spectral overlaps.