Section of Immunobiology, Department of Internal Medicine and Yale Cancer Center

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BD LSR II Green

Special Configuration

Multilaser multiparameter analysis cytometer

Four lasers, 12 colors

Digital data collection using FACS DIVA software

Automatic compensation (compensation controls are still needed)

405 nm (Violet) : Detector A: AmCyan, CFP; Detector B: Pacific Blue, Marina Blue, Alexa Fluor 405, BFP

488 nm (Blue) : Detector A: PerCP; Detector B: FITC,GFP, CFSE, Alexa 488; Detector C: SSC

532 nm (Green) Detector A: PE-Cy7; Detector B: PE-Cy5.5; Detector C: PE-Cy5; Detector D: Alexa 568, PI, Texas Red-PE; Detector E: PE, Alexa 532

635 nm (Red): Detector A: APC-Cy7; Detector B: Alexa 680, Alexa 700; Detector C: APC, Alexa 647;