Cell Sorting
In TAC S617 are 3 high-speed sorters: a BD FACSVantage SE, a BD Aria, and a Dako MoFlo. Cells can be sorted at the rate of 20K/sec. at 99+% purity, using a variety of commonly used fluorochromes, eg. FITC, PE, PE-Cy5, APC, APC-CY7, PE-CY7, CY5, Alexa680 and PI. The MoFlo has 7-color, Vantage SE 6-color and the Aria has 10-color capability.

Cells can be sorted into 5 or 15ml tubes or into various plates as single or multiple cells/well or onto microscope slides for analysis. In addition, the sorters are capable of simultaneous 4-way sorting. FACS Vantage SE and FACS Aria are also equipped with aerosol management systems making them suitable for sorting live human, primate or other potentially biohazardous cells.

The SE and MoFlo have dedicated operators (Tom Taylor and Gouzel Tokmoulina) who will help with experimental design and running your samples. The Aria can be user operated for sorting and multicolor analysis by trained individuals or can be operated by the facility; contact Geoff Lyon or Zhao Zhao about using the Aria or having them run your samples.

Users must come to the facility to reserve time on sorters.

FACS Analysis
There are 8 user-operated analyzers (LSR II, LSRII Green, LSRII TAC5, five FACSCaliburs and a FACScan). The Caliburs can analyze up to 4 colors with 488 nm and 633 nm excitation lasers while the FACScan can analyze 3 colors from a single 488 nm laser. LSR II and LSRII Green have a capability of 12 colors. Machines are checked daily for optimal performance and Ewa Menet is available during working hours to troubleshoot and can arrange training by appointment. Internet sign up for analysis time is available from any computer via the internet at www.schedulebook.com.

New Satellite facility
opened in Amistad building and is located in room 416. FACS Aria sorter has 4 lasers: 355 nm, 405 nm , 488 nm and 635 nm. BD LSR II is equipped also with 4 lasers:488 nm blue, 635 nm red, 532 nm green, and a 355 nm UV laser. Geoff Lyon is in charge of both cytometers and can be contacted to schedule the sort.