Page designed and maintained by Octavian Henegariu (Email: or ). WARNING: The information provided in these pages is copyrighted and is intended for individual use only. No parts of this work (text, tables or pictures) may be commercialized, published or otherwise reproduced without the written consent of the author. Ref: Laboratory Investigation. 2001 Apr;81(4):483-91. Get article in PDF format here PCR | dUTP label | FISH | FISH guide| CCK | Slide prep | CM-FISH | TM-FISH | mArrays | Home TM-FISH Table 1 Mouse and human probe combinations for TM-FISH analysis Chr Clone name Mouse TM-FISH Chr Clone name Human TM-FISH 1/5 and 2/3 combinations Chr Human TM-FISH 3/3 combinations Set # F R N B D F R N B D D N B 1c 45C1 1 1 1 1p 13p11 1 1 1 1p 1 1 1 1t 39L15 1q 160h23 1q 2c 49N22 2 2 2 2p 68j13 2 2 2 4p 4 2t 20L10 2q 210e4 4q 3c 33D15 3 3 3 3p B47a2 3 3 3 9p 9 9 3t 443K17 3q 196f4 9q 4c 39B18 4 4 4p 36p21 4 4 10p 10 #1 4t 362D3 4q cT55 10q 5c 48H24 5 5 5 5p 114j18 5 13q 13 13 5t 40J4 5q 240g13 17p 17 6c 45K20 6 6p 36i2 6 6 6 17q 6t 51F24 6q 57h24 20p 20 7c 43A19 7 7 7p 164d18 7 7 20q 7t 100O21 7q 3k23 22q 22 22 8c 20H8 8 8 8p 63m14 8 8 2p 2 2 8t 38K2 8q 2053b3 2q 9c 23D13 9 9 9p 34h2 9 9 5p 5 9t 55J6 9q 112n13 5q 10c 47K7 10 10 10p 305f4 10 10 7p 7 10t 26C12 10q 2136a1 7q 11c 39E20 11 11 11p 2209a2 11 11 8p 8 11t 434L24 11q 2072c1 8q #2 12c 47N14 12 12 12p 90i5 12 12 11p 11 11 12t 34I19 12q 221k18 11q 13c 50K9 13 13 13q 85a10 13 13 15q 15 15 15 13t 38I3 14q 2006a1 14 16p 16 16 14c 429N17 14 15q 154p1 15 15 16q 14t 48O2 16p 119l16 16 16 16 19p 19 15c 43G16 15 15 16q 240g10 19q 15t 62I2 17p 2111b1 17 17 3p 3 16c 50I1 16 16 16 17q 362k4 3q 16t 43D12 18p 52m11 18 6p 6 6 17c 65C22 17 17 18q 2050a6 6q 17t 50F18 19p F20643 19 12p 12 12 18c 53M14 18 19q F21283 12q #3 18t 51B23 20p 2005a4 20 14q 14 14 19c 26B5 19 20q 204a16 18p 18 19t 49P14 21q 63h24 21 21 21 18q Xc 51A6 X 22q 99k24 22 22 22 21q 21 21 21 Xt 23H12 XYp 98c4 xy xy xy XYp xy XYq c8.1/2 XYq "Chr" = chromosome number; "c", "t" for mouse clones = the centromeric or telomeric end of the chromosome. "p", "q" for human clones = indicate the p and q arm of the chromosome. F = FITC; R = R6G; N = DNP; B = BIO; D = DIG. Numbers within the table indicate the fluors or haptenes with which the probes for the respective chromosome were labeled. We used numbers, so that chromosome identification is easier. Italic characters of some human clone names indicate the cosmid probes. All other human probes were P1, PACs. When human probes are used according to the 2/3 approach, FITC is replaced by BIO, and R6G by DIG. The probes in these two columns are then hybridized on one area of the slide, whereas the rest of the human clones (the ones always labeled by BIO, DIG, DNP) are hybridized on another area of the slide. Note: to differentiate the chromosomes, information from both hybridizations needs to be combined. #1-#3 = the three probe mixes used for the 3/3 TM-FISH combination. Probes corresponding to chromosomes 4 and 20 (mix #1) and chromosomes 7 and 19 (mix #2), respectively, are differentiated by the size of the fluorescent signal, not by combinatorial labeling.