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(Ref: Biotechniques 2001, Vol 31, p246-250. Get article here)


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FiberFISH - Fig 2

Fig. 2. Size distribution of 32 lambda phage DNA fibers, measured from five different microscopic fields (using 100x objective).  Because of random breakages in the DNA molecules, only the longest of these fibers were considered when measuring the average size of an intact lambda molecule (in this case, only the 15 fibers between 156-161 pixels). The average size of an intact DNA molecule was 159 pixels, or 130% over-stretching. This number was subsequently verified by checking other molecules with known lengths.