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Table 6 (FISH guide)
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Table 6. Type of hybridization buffers.
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Regular buffer |
Concentrated buffer |
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50% FA 2x SSC 1x phosphate buffer* 10% dextran sulfate |
75-80% FA 2x SSC 1x phosphate buffer* 15-20% dextran sulfate |
*10 x phosphate buffer = 0.5M 5:1 sodium phosphate dibasic: sodium phosphate monobasic (final pH~7.0)