|
|
Page designed
and maintained by Octavian Henegariu (Email:
or ). |
|
WARNING:
The information provided in these pages is copyrighted and is intended
for individual use only. No parts of this work (text, tables or
pictures) may be commercialized, published or otherwise reproduced
without the written consent of the author. |
Table 1 (FISH guide)
TOPICS:
| 1
| 2
| 3
| 4
| 5
| 6
| 7
| 8
| 9
| 10
| 11
| 12
| 13
| 14
| 15
| 16
| 17
| 18
| 19
| 20
| 21
| 22
| 23
FIGURES: | 1
| 2
|
3 |
4 |
5 |
6 |
7
| 8
| 9
| 10
| 11
| TABLES:
| 1
| 2
| 3
| 4
| 5
| 6
| 7
Table 1. DNA probe labeling procedures.
|
NICK TRANSLATION |
PCR LABELING |
|
1 ug DNA (20 ng/µl final conc.) 5 µl (10x) nick translation buffer * 5 µl (100mM=10x) -mercaptoethanol 2.5µl (1mM=20x) each dACG 5µl (0.325/0.175 mM=10x) dTTP/dUTP 5µl (1µg/µl=10x) DNase (Sigma), 1µl (10U/µl) E. Coli polymerase ultrapure sterilized water to 50 µl. |
Template DNA (various amounts) 2.5-3.5µl (10x) PCR buffer 0.15µl (33.3mM) each dACG 0.7µl (5mM) dTTP 1.6µl (1mM) dUTP 0.1-1µl (50µM) primer(s) 0.2-0.4µl (5U/µl) Taq polymerase ultrapure sterilized water to 25 µl |
* = any E.coli polymerase buffer
dUTP = labeled dUTP deoxynucleotide.