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Figure 4 (slideprep)
Slide preparation figures:
Fig1
| Fig2
| Fig3
| Fig4
| Fig5
| Fig6
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Legend: Fig. 4. Pellet resuspended in 1:1 methanol:acetic acid (column a ), 6:1 methanol:acetic acid (column b) and 3:1 ethanol:acetic acid (column c). In row #1, no water vapors, slides dried on the cold end of the metal plate (room temperature); #2, slides exposed to water vapors, then dried at room temperature; #3, no water vapors, slide dried on the hot end of the metal plate; #4, slides exposed to water vapors, dried on the hot end of the metal plate.
The best spreading and cleanest chromosome preparations are achieved in #1 and #4.
