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Figure 3 (slideprep)
Slide preparation figures:
Fig1
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Legend: Fig. 3a-3ee: illustrates the processes taking place during chromosome spreading, as examined at the microscope using a 10x objective. In 3a-3e series, the pellet was placed directly on the slide, whereas in the 3aa-3ee series the cell suspension was dropped from about 1m distance. The letters correspond to the arbitrary five steps described in the text. Microscopically and macroscopically there is no difference in chromosome spreading between the two techniques. The drying technique is much more important.
3f-3i. (compare also with fig. 4). Cells resuspended in 3:1 methanol:acetic acid were dropped on slides and dried as follows: 2f, no water vapors, slide dried on the cold end of the metal plate (room temperature); 2g, slide exposed to water vapors, then dried at room temperature; 2h, no water vapors, slide dried on the hot end of the metal plate; 2i, slide exposed to water vapors, dried on the hot end of the metal plate.
