Kidd Lab 

MspI in HOXB4; Clone pH 2-1-3 Bg 2

 Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

 Primer 1.      BG2FIN2 '5-AGG ATG TGT GAG AGG CTG G          100 ng/reaction
 Primer 2.      BG2RINT '5-GCA CAA AAG GAG GAA GGG TC       100 ng/reaction

 dNTPs: Final Concentration of 200 µM each

 Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris HCI pH 8.4

 Taq DNA Polymerase:     0.5 Units/reaction volume of 25 µl
                                      (Perkin-Elmer Amplitaq DNA polymerase 5U/µl, Cat No. N808-0145)

 PCR Cycling Profile:        94^oC (5')
                                      94^oC (30"), 60^oC (30"), 72^oC (1.0')      × 30 cycles

 Product Size:
                                      MspI
                           560 bp --------->350 bp + 210 bp   (+/+ individual)

Ogura, T., C.M. Castiglione, A.J. Pakstis, and K.K. Kidd, 1991. An MspI polymorphism for the HOX2F gene. Nucleic Acids Research 19:1716.

 [Note: RFLP alleles seen by Southern blotting: 2 kb; 1.8 kb; 3.0 kb (rare, seen only in Africa).]
 

Kidd Lab Histograms for Allele Frequencies