Kidd Lab 

Xba I

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1. APOBXB1:     5'-AAA TAA CCT TAA TCA TCA ATT GGT 3'     100ng/reaction
Primer 2. APOBXB2:     5'-GGT TCT TAG CAG CAA GAG TCC 3'          100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC, 5'
                              95^oC (30"), 60^oC (30"), 72^oC (30")  × 30 cycles

Product size:               XbaI
                        230 bp----------> 230 and 160 + 70 bp in heterozygote

Boerwinkle, E., S.S.Lee, R. Butler, VN Schumaker, and L. Chan, 1990. Rapid typing of apolipoprotein B DNA polymorphisms by DNA amplification. Athero 81:225-232.
 

Kidd Lab Histograms for Allele Frequencies