Kidd Lab 

TaqI SNP site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     TKTQCOMP:    5'-TGG ATT CAC GCC CTC TTG TTT C-3'         100 ng/reaction
Primer 2.     TKTAQ3:          5'-CCC AGA CAG GAA GGC AGA TGA-3'       100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.15 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 57^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                  TaqI
                    703 bp ----------------> 517 bp + 186 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies