Kidd Lab 

BstEII SNP site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     TKTAQF:   5'-CAA GAG GCT CGG CAC AGA G-3'              100 ng/reaction
Primer 2.     TKTAQR:   5'-GAA ACA AGA GGG CGT GAA TCC A-3'       100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.15 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 62^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                BstEII
                    799 bp ----------------> 420 bp + 379 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies