Kidd Lab 

40-bp VNTR located in 3'-UTR (422 bp downstream from G2319A MspI polymorphism)

Template: Genomic DNA 100-200 ng/reaction volume of 15 µl

Primer 1.    DATVNTRF2:    5'-GGT GTA GGG AAC GGC CTG AGA G-3'           Final Concentration 0.2 µM
Primer 2.    DATVNTRR:    5'-CTT CCT GGA GGT CAC GGC TCA AGG-3'         Final Concentration 0.2 µM

dNTPs: Final Concentration 200 µM

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4
DMSO: Final Concentration 5%

Taq DNA Polymerase: 1 Unit/reaction volume of 15 µl
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              94ºC (30"), 62ºC (30"), 72ºC (30")  x 30 cycles

Product Size: 2% agarase gel, EtBr stain
 

564 bp	12 repeats
524 bp	11 repeats
484 bp	10 repeats
444 bp	9 repeats
...	...
364 bp	7 repeats
324 bp	6 repeats
...	...
...	...
199 bp	3 repeats

 
 
 

Kidd Lab Histograms for Allele Frequencies