Kidd Lab 

TaqI site (SNP in intron 19)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     RET3TAQF:    5'-GTT CTA TAA AAC ATT CTC TAA CG-3'              100 ng/reaction
Primer 2.     RET3TAQR:   5'-CAT CAG GGG TAG CGA GGT TG-3'                  100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 µl
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              94^oC (30"), 56^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                  TaqI
                   501 bp  -----------> 222 bp + 279 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies