Kidd Lab 

CA_(n) site (located in intron 5)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.    Retin5caf:          5'-CAG GGC CTT GGT AAT GTA GAC-3'                        50 ng/reaction
Primer 2.    Retin5car:          5'-TTT TAC AGA GTT TCG TTT TTT GGG-3'                    50 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 1.0 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (2')
                              95^oC (30"), 62^oC (30"), 75^oC (30")  × 35 cycles
                             72^oC (10')

Product size:  107, 109, 111, 113, 115, 117, 119, 121 bp

Reference:Pasini et al., 1995. The physical map of the human RET proto-oncogene   Oncogene 11: 1737-1743
 
 

Kidd Lab Histograms for Allele Frequencies