Kidd Lab 

(CA)_n STRP (sTCLl-2) (located past 3' end RET gene toward D10S94)

Template: Genomic DNA 50-100 ng/reaction volume of 25 µl

Primer 1.     RET1*:     5'-CCA GAC TCT CAA AGA CCA GG-3'          10 pM/reaction
Primer 2.     RET2:      5'-CAT AAT ACT GGC CTA TAG-3'                  20 pM/reaction
                  * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

BSA:   Final Concentration 1.1 ug/ul

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  95^oC (5')
                               95^oC (30"), 50^oC (30"), 72^oC (1')  × 30 cycles

Product size: Ranges from 150-180 bp ( Run on a 6% polyacrylamide, 8M urea gel )

Reference:
Lairmore et al., 1993. PNAS 90: 492-496
 
 

Kidd Lab Histograms for Allele Frequencies