Kidd Lab 

Alu insertion site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.    PLAT.A:    5'-GTG AAA AGC AAG GTC TAC CAG-3'        100 ng/reaction
Primer 2.    PLAT.B:    5'-GAC ACC GAG TTC ATC TTG AC-3'            100 ng/reaction

dNTPs: Final Concentration 200 µM

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 µl
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (1'), 60^oC (1'), 72^oC (1')  × 30 cycles

Product Size: 570 bp and 260 bp for heterozygous (+/-) sample

Reference:
Friezner-Degen, SJ, B Rajput, E. Reich, 1986.  The human tissue plasminogen activator gene.  J. Biol. Chem. 261: 6972-6985.
Tishkoff  SA, Ruano G, Kidd JR, Kidd KK, 1996. Distribution and frequency of a polymorphic Alu insertion at the plasminogen activator locus in humans. Hum. Genet.97: 759-764.
 
 

Kidd Lab Histograms for Allele Frequencies