Kidd Lab 

XmnI site (located about 2 kb 3' of the MspI site in intron 8)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     PAHXMN1:    5'-CTG TAC TTG TAA GAT GCA GC-3'         100 ng/reaction
Primer 2.     PAHXMN2:    5'-ACT GTC CCA AGC AAT CAA AG-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  95^oC (5')
                               95^oC (30"), 58^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                 XmnI
                   205 bp ---------------> 110 bp + 95 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies