Kidd Lab 

TaqI (A) site (7.18 kb downstream of exon 6)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     NGFRTQAF1:    5'-GAC ACT GGT TAT ACT CTA GTC C-3'         100 ng/reaction
Primer 2.     NGFRTQAR1:    5'-CAG TGG CTT GCA GAT GAT TC-3'             100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 60^oC (30"), 72^oC (30")  × 30 cycles
                               72^oC (7')

Product size:
                                 Taq I
                   423 bp ------------------> 82 bp + 341 bp (in heterozygote)
 
 

Kidd Lab Histograms for Allele Frequencies