Kidd Lab 

StyI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     NCAMRS4F:      5'-GTA TAA GTG GTC TCC CCT CTC-3'            50 ng/reaction
Primer 2.     NCAMRS4R:      5'-GAG AAG TGA TCA CAG CAG CAG-3'         50 ng/reaction
(NOTE:THE PRIMER AMOUNT!)

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (4')
                              94^oC (1'), 63^oC (30"), 72^oC (1.5')  × 35 cycles

Product size:
                                StyI
                   1625 bp--------------> 1625 bp and 1075 bp + 550 bp (in heterozygote)
 
 

Kidd Lab Histograms for Allele Frequencies